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Product Info Summary
| SKU: | PB9497 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NQO1 Antibody Picoband®
SKU/Catalog Number
PB9497
PB0526 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NQO1 Antibody Picoband® catalog # PB9497. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NQO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9497)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NQO1, different from the related mouse and rat sequences by five amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9497 is reactive to NQO1 in Human, Rat
Observed Molecular Weight
31 kDa
Calculated molecular weight
30.9 kDa
Background of NQO1
This gene is a member of the NAD (P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. And this FAD-binding protein forms homodimers and reduces quinones to hydroquinones. In addition, this protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9497 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: human SH-SY5Y whole cell, human MCF-7 whole cell, human Hela whole cell, rat RH35 whole cell
Validation Images & Assay Conditions
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RSBQD activated PI3K/AKT/Nrf2 signaling pathway in exercise fatigued mice (n = 10). A Hepatic GSH-Px activity. B Hepatic SOD activity. C Hepatic MDA content. D – F The relative mRNA levels of Sod1 ( D ), Nrf2 ( E ), and Ho-1 ( F ). G Representative images of Western blot. H – K Relative protein levels of KEAP1 ( H ), NRF2 ( I ), HO-1 ( J ), and NQO1 ( K ). ( L , M ) The ratio of p-PI3K/PI3K L and p-AKT/AKT M . * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Index in PubMed under a CC BY license. PMID: 39501343
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Western blot analysis of NQO1 using anti-NQO1 antibody (PB9497).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: rat RH35 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (Catalog # PB9497) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 31 kDa. The expected band size for NQO1 is at 31 kDa.
Specific Publications For Anti-NQO1 Antibody Picoband® (PB9497)
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1 Customer Q&As for Anti-NQO1 Antibody Picoband®
Question
We are currently using anti-NQO1 antibody PB9497 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?
R. Evans
Verified customer
Asked: 2015-01-23
Answer
The anti-NQO1 antibody (PB9497) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-01-23
