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Product Info Summary
|Reactive Species:||Human, Rat|
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Anti-NQO1 Antibody Picoband™
Boster Bio Anti-NQO1 Antibody Picoband™ catalog # PB9497. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NQO1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9497)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the C-terminus of human NQO1 (242-274aa EVQDEEKNKKFGLSVGHHLGKSIPTDNQIKARK), different from the related mouse and rat sequences by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PB9497 is reactive to NQO1 in Human, Rat
PB9497 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of NQO1 using anti-NQO1 antibody (PB9497).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: Rat Lung Tissue Lysate at 50ug,
Lane 3: HELA Whole Cell Lysate at 40ug,
Lane 4: A549 Whole Cell Lysate at 40ug,
Lane 5: MM231 Whole Cell Lysate at 40ug,
Lane 6: SW620 Whole Cell Lysate at 40ug,
Lane 7: 22RV1 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 antigen affinity purified polyclonal antibody (Catalog # PB9497) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1 at approximately 31 kDa. The expected band size for NQO1 is at 31 kDa.
Gene/Protein Information For NQO1 (Source: Uniprot.Org, NCBI)
NAD(P)H dehydrogenase [quinone] 1
NAD(P)H dehydrogenase (quinone) family
Azoreductase; DHQU; DIA4; DIA4azoreductase; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase-4; Dioxin-inducible 1; DTD; DTDEC 220.127.116.11; DT-diaphorase; Menadione Reductase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NMOR1; NMOR1diaphorase-4; NQO1; NQO-1; Phylloquinone reductase; QR1; QR1NMORI; Quinone Reductase 1 NQO1 DHQU, DIA4, DTD, NMOR1, NMORI, QR1 NAD(P)H quinone dehydrogenase 1 NAD(P)H dehydrogenase [quinone] 1|DT-diaphorase|NAD(P)H dehydrogenase, quinone 1|NAD(P)H:Quinone acceptor oxidoreductase type 1|NAD(P)H:menadione oxidoreductase 1|NAD(P)H:quinone oxidoreductase 1|NAD(P)H:quinone oxireductase|azoreductase|diaphorase (NADH/NADPH) (cytochrome b-5 reductase)|diaphorase-4|dioxin-inducible 1|menadione reductase|phylloquinone reductase|quinone reductase 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on NQO1, check out the NQO1 Infographic
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In this infographic you will see the following information for NQO1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for PB9497
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