Product Info Summary
SKU: | A14688 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-B3GALT1 Antibody
SKU/Catalog Number
A14688
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-B3GALT1 Antibody (Catalog # A14688). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B3GALT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14688)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human B3GALT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14688 is reactive to B3GALT1 in Human, Mouse
Applications
A14688 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
37.993kDa
Background of B3GALT1
Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.
Bardoni A., FEBS Lett. 451:75-80(1999).
Liu Y., Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., Nature 434:724-731(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HepG2 cells, Jurkat cells, COLO cells and HUVEC cells, using B3GALT1 antibody A14688.
Protein Target Info & Infographic
Gene/Protein Information For B3GALT1 (Source: Uniprot.org, NCBI)
Gene Name
B3GALT1
Full Name
Beta-1,3-galactosyltransferase 1
Weight
37.993kDa
Superfamily
glycosyltransferase 31 family
Alternative Names
beta-1,3-galactosyltransferase 1; Beta-1,3-GalTase 1; beta3GalT1; beta-3-galt1; beta3Gal-T1; EC 2.4.1; EC 2.4.1.-; EC 2.4.1.62; MGC126594; UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1; UDP-galactose:beta-N-acetyl-glucosamine-beta-1,3-galactosyltransferase 1 B3GALT1 beta3Gal-T1 beta-1,3-galactosyltransferase 1 beta-1,3-galactosyltransferase 1|UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1|UDP-galactose:beta-N-acetyl-glucosamine-beta-1,3-galactosyltransferase 1|beta-1,3-GalTase 1|beta3GalT1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on B3GALT1, check out the B3GALT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for B3GALT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B3GALT1 Antibody (A14688)
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No publications found for A14688
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-B3GALT1 Antibody
Question
Does anti-B3GALT1 antibody A14688 work on pig WB with placenta?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Our lab technicians have not tested anti-B3GALT1 antibody A14688 on pig. You can run a BLAST between pig and the immunogen sequence of anti-B3GALT1 antibody A14688 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-12
Question
We are currently using anti-B3GALT1 antibody A14688 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
The anti-B3GALT1 antibody (A14688) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-14
Question
Do you have a BSA free version of anti-B3GALT1 antibody A14688 available?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-B3GALT1 antibody A14688 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-13
Question
Please see the WB image, lot number and protocol we used for caudate nucleus using anti-B3GALT1 antibody A14688. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-16
Question
I am looking for to test anti-B3GALT1 antibody A14688 on human caudate nucleus for research purposes, then I may be interested in using anti-B3GALT1 antibody A14688 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Johnson
Verified customer
Asked: 2016-02-18
Answer
The products we sell, including anti-B3GALT1 antibody A14688, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-02-18
Question
Is this A14688 anti-B3GALT1 antibody reactive to the isotypes of B3GALT1?
J. Roberts
Verified customer
Asked: 2014-09-03
Answer
The immunogen of A14688 anti-B3GALT1 antibody is Synthesized peptide derived from internal of human B3GALT1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-09-03
Question
Does anti-B3GALT1 antibody A14688 work for WB with caudate nucleus?
S. Kulkarni
Verified customer
Asked: 2014-03-07
Answer
According to the expression profile of caudate nucleus, B3GALT1 is highly expressed in caudate nucleus. So, it is likely that anti-B3GALT1 antibody A14688 will work for WB with caudate nucleus.
Boster Scientific Support
Answered: 2014-03-07