Click image to see more details
Product Info Summary
| SKU: | A30572 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-B3GALTL B3GLCT Antibody
SKU/Catalog Number
A30572
Size
100ul
Form
Liquid
Description
Boster Bio Anti-B3GALTL B3GLCT Antibody (Catalog# A30572). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B3GALTL B3GLCT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30572)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human B3GALTL. Immunogen sequence location: 449-498
Reactive Species
A30572 is reactive to B3GLCT in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
56.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30572 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using β-1,3-Gal-TL Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using B3GALTL Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HUVEC, MCF-7, Jurkat, and HepG2 cells, using B3GALTL Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-B3GALTL B3GLCT Antibody (A30572)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-B3GALTL B3GLCT Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-B3GALTL antibody A30572. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
Do you have a BSA free version of anti-B3GALTL antibody A30572 available?
B. Rodriguez
Verified customer
Asked: 2020-01-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-B3GALTL antibody A30572 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-31
Question
See attached the WB image, lot number and protocol we used for female gonad using anti-B3GALTL antibody A30572. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-19
Question
Is this A30572 anti-B3GALTL antibody reactive to the isotypes of B3GLCT?
R. Moore
Verified customer
Asked: 2017-07-24
Answer
The immunogen of A30572 anti-B3GALTL antibody is A peptide derived from human B3GALTL. Immunogen sequence location: 449-498. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-24
Question
I see that the anti-B3GALTL antibody A30572 works with WB, what is the protocol used to produce the result images on the product page?
P. Wu
Verified customer
Asked: 2016-12-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2016-12-16
Question
We are currently using anti-B3GALTL antibody A30572 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
W. Parker
Verified customer
Asked: 2013-02-25
Answer
The anti-B3GALTL antibody (A30572) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-25
