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- Table of Contents
CD276 antibody (M02220-2) performs well in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
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Anti-B7H3 CD276 Monoclonal Antibody [2H5]
CD276 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Mouse | ELISA, Flow Cytometry, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
B7-H3 antibody can be used for detection of B7-H3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-B7H3 CD276 Monoclonal Antibody [2H5]
View all CD276 Antibodies
View all CD276 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
B7-H3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Mouse
Contents
B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Clonality
Monoclonal
Clone Number
Clone: 2H5
Isotype
IgG1,k
Immunogen
B7-H3 antibody was raised against the extracellular domain of human B7-H3
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
57.2 kDa
Background of CD276
CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.
Cite This Product
Anti-B7H3 CD276 Monoclonal Antibody [2H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02220-2)
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Product Promise
We promise Anti-B7H3 CD276 Monoclonal Antibody [2H5] performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M02220-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026