Anti-B7H3 CD276 Monoclonal Antibody [2H5]

Product Name

Anti-B7H3 CD276 Monoclonal Antibody [2H5]

View all B7-H3/CD276 Antibodies

SKU/Catalog Number

M02220-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-B7H3 CD276 Monoclonal Antibody [2H5] (Catalog # M02220-2). Tested in ELISA, WB, IHC-P, ICC, IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

B7-H3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-B7H3 CD276 Monoclonal Antibody [2H5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02220-2)

Host

Mouse

Contents

B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 2H5

Isotype

IgG1,k

Immunogen

B7-H3 antibody was raised against the extracellular domain of human B7-H3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.

Reactive Species

M02220-2 is reactive to CD276 in Human

Applications

M02220-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

57.235kDa

Background of B7-H3/CD276

CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

B7-H3 antibody can be used for detection of B7-H3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and Immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL. Flow cytometry at 1 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.

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Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.

Green: B7-H3 Antibody [2H5] (RF16091)
Blue: DAPI staining

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Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.

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Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.

Gene/Protein Information For CD276 (Source: Uniprot.org, NCBI)

Alternative Names

B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule CD276 4Ig-B7-H3, B7-H3, B7H3, B7RP-2 CD276 molecule CD276 antigen|B7 homolog 3|costimulatory molecule

For more info on CD276, check out the CD276 Infographic

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In this infographic, you will see the following information for CD276: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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