Click image to see more details
Product Info Summary
| SKU: | M02220 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-CD276/B7 H3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02220
BM5154 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD276/B7 H3 Rabbit Monoclonal Antibody catalog # M02220. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD276/B7 H3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02220)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABGB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD276
Reactive Species
M02220 is reactive to CD276 in Human, Mouse
Observed Molecular Weight
100 kDa
Calculated molecular weight
57.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02220 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
FC 1:20
Positive Control
WB: 293T cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD276 expression in 293T cell lysate.
Specific Publications For Anti-CD276/B7 H3 Rabbit Monoclonal Antibody (M02220)
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Customer Q&As
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7 Customer Q&As for Anti-CD276/B7 H3 Rabbit Monoclonal Antibody
Question
Will anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220 work for IP with liver?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
According to the expression profile of liver, CD276 is highly expressed in liver. So, it is likely that anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220 will work for IP with liver.
Boster Scientific Support
Answered: 2019-08-15
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-21
Question
Is there a BSA free version of anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220 available?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-04-12
Question
I see that the anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220 works with IP, what is the protocol used to produce the result images on the product page?
C. Anderson
Verified customer
Asked: 2018-11-23
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-11-23
Question
Our lab want to know about to test anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220 on human liver for research purposes, then I may be interested in using anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
The products we sell, including anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-12
Question
Can you help my question with product M02220, anti-CD276/B7 H3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Evans
Verified customer
Asked: 2017-05-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02220 anti-CD276/B7 H3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-24
Question
I have attached the WB image, lot number and protocol we used for liver using anti-CD276/B7 H3 Rabbit Monoclonal antibody M02220. Please let me know if you require anything else.
A. Kulkarni
Verified customer
Asked: 2014-11-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-03
