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Product Info Summary
| SKU: | A01257-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BAFF TNFSF13B Antibody
SKU/Catalog Number
A01257-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BAFF TNFSF13B Antibody (Catalog # A01257-1). Tested in ELISA, WB, ICC, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BAFF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BAFF TNFSF13B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01257-1)
Host
Rabbit
Contents
BAFF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-BAFF antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF. The immunogen is located within the last 50 amino acids of BAFF.
Reactive Species
A01257-1 is reactive to TNFSF13B in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
31.2 kDa
Background of TNFSF13B
Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF-κB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01257-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.25-1 μg/mL; ICC: 1 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human HL60 Cell Lysate (H) and Mouse Spleen Lysate (M)
Loading: 15 μg of lysates per lane.
Antibodies: BAFF A01257-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: BAFF A01257-1 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Western Blot Validation
with Recombinant Protein
Loading: 30 ng of human BAFF recombinant protein per lane.
Antibodies: BAFF A01257-1 (Lane 1: 0.25 μg/mL; Lane 2: 0.5 μg/mL and Lane 3: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Observed at around 18kD.
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Immunocytochemistry Validation of BAFF in HL60 Cells
Immunocytochemical analysis of HL60 cells using anti-BAFF antibody (A01257-1) at 1 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of BAFF in Human Spleen Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling BAFF with A01257-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Regulated Expression Validation of BAFF in
Myeloma Patients (Tai et al., 2006)
Immunoblot analysis was performed to monitor protein expression of BAFF with anti-BAFF antibodies in multiple myeloma cells with or without BMSCs. BAFF expression in cocultures at 8hr or 24hr was up-regulated by ~3.5-fold relative to BMSCs alone.
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Immunohistochemistry Validation of BAFF in Thyroid of Patients with Graves' Diseases (Campi et al., 2015)
BAFF expression detected by anti-BAFF antibodies (A01257-1) was remarkably increased in thyrocytes from multinodular goiter (C) compared with either Hashimoto's thyroiditis (E) or Graves' disease (G) while no staining was found in normal thyroid tissue (A).
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Immunohistochemistry Validation of BAFF in Murine Cardiac Transplants at Rejection (Ye et al., 2004)
BAFF expression detected by anti-BAFF antibodies (A01257-1) was upregulated in intragraft leukocytes due to rejection at 7 days after heart transplant.
Specific Publications For Anti-BAFF TNFSF13B Antibody (A01257-1)
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