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Product Info Summary
| SKU: | PB9754 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Bag3 Antibody Picoband®
SKU/Catalog Number
PB9754
PB0801 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bag3 Antibody Picoband® catalog # PB9754. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bag3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9754)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Bag3 recombinant protein (Position: H100-N561). Human Bag3 shares 84.1% amino acid (aa) sequence identity with mouse Bag3.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9754 is reactive to BAG3 in Human
Observed Molecular Weight
80 kDa
Calculated molecular weight
61.6 kDa
Background of BAG3
BAG family molecular chaperone regulator 3 (BAG3) is a member of a conserved family of cyto-protective proteins that bind to and regulate Hsp70 family molecular chaperones. BAG3 mutations are responsible for familial dilated cardiomyopathy. BAG3 polymorphisms are also associated with sporadic forms of the disease together with HSPB7 locus. In muscle cells, BAG3 cooperates with the molecular chaperones Hsc70 and HspB8 to induce the degradation of mechanically damaged cytoskeleton components in lysosomes. This process is called chaperone-assisted selective autophagy (CASA) and is essential for maintaining muscle activity in flies, mice and men.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9754 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human A431 whole cell, human 293T whole cell, human U251 whole cell, human U87 whole cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Bag3 using anti-Bag3 antibody (PB9754).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human U87 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag3 antigen affinity purified polyclonal antibody (Catalog # PB9754) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for Bag3 at approximately 80 kDa. The expected band size for Bag3 is at 62 kDa.
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IHC analysis of Bag3 using anti-Bag3 antibody (PB9754).
Bag3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Bag3 Antibody (PB9754) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Bag3 Antibody Picoband® (PB9754)
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1 Customer Q&As for Anti-Bag3 Antibody Picoband®
Question
We are currently using anti-Bag3 antibody PB9754 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The anti-Bag3 antibody (PB9754) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-14
