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Product Info Summary
| SKU: | AZF1QGM6 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Zebrafish BAG5 Antibody Picoband®
SKU/Catalog Number
AZF1QGM6
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish BAG5 Antibody Picoband® catalog # AZF1QGM6. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20 ℃ for one year from date of receipt. After reconstitution, at 4 ℃ C for one month. It can also be aliquotted and stored frozen at -20 ℃ for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish BAG5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1QGM6)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish BAG5 recombinant protein (Position: K453-Y511).
Reactive Species
AZF1QGM6 is reactive to BAG5 in Zebrafish
Observed Molecular Weight
50 kDa
Background of BAG5
BAG family molecular chaperone regulator 5 is a protein that in humans is encoded by the BAG5 gene. The protein encoded by this gene is a member of the BAG1-related protein family. Bag5 is a negative regulator of both Hsp70 and parkin function that sensitizes dopaminergic neurons to injury-induced death and thus promotes neurodegeneration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZF1QGM6 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Positive Control
WB: zebrafish tissue
IHC: zebrafish heart tissue
Validation Images & Assay Conditions
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Western blot analysis of Zebrafish BAG5 using anti-Zebrafish BAG5 antibody (AZF1QGM6).
Electrophoresis was performed on a 10% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zebrafish BAG5 antigen affinity purified polyclonal antibody (AZF1QGM6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Zebrafish BAG5 at approximately 50 kDa. The expected band size for Zebrafish BAG5 is at 57 kDa.
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IHC analysis of Zebrafish BAG5 using anti-Zebrafish BAG5 antibody (AZF1QGM6).
Zebrafish BAG5 was detected in paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Zebrafish BAG5 Antibody (AZF1QGM6) overnight at 4°C.Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish BAG5 Antibody Picoband® (AZF1QGM6)
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