Anti-BCAS3 Antibody

Product Name

Anti-BCAS3 Antibody

View all BCAS3 Antibodies

SKU/Catalog Number

A07120-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BCAS3 Antibody (Catalog # A07120-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

BCAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BCAS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07120-1)

Host

Rabbit

Contents

BCAS3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BCAS3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human BCAS3. The immunogen is located within amino acids 50 - 100 of BCAS3.

Reactive Species

A07120-1 is reactive to BCAS3 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

101.2 kDa

Background of BCAS3

The Breast carcinoma amplified sequence (BCAS3) gene is localized to 17q23, a region that is often amplified in breast cancers. Copy number gains at this region have also been reported in other tumors such as brain, lung, liver, testis and bladder. BCAS3 is thought to be an estrogen receptor (ER)-alpha co-activator that acts through PELP1, another ER-alpha that in turn activates BCAS3 expression. This may set up a positive feedback loop leading to ER-a signal amplification in the cell which may play a significant role in breast cancer. BCAS3 expression has also been observed in embryonic stem cells and vascular precursors, suggesting that it may also play a role in embryogenesis and tumor angiogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07120-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BCAS3 antibody can be used for detection of BCAS3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BCAS3

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