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Product Info Summary
| SKU: | A07120 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-BCAS3 Antibody
SKU/Catalog Number
A07120
Size
100μl
Form
Liquid
Description
Boster Bio Anti-BCAS3 Antibody catalog # A07120. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BCAS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07120)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from BCAS3 . at AA range: 660-740
Reactive Species
A07120 is reactive to BCAS3 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
101.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07120 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:5000
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of 293 cells using BCAS3 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
Specific Publications For Anti-BCAS3 Antibody (A07120)
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Customer Q&As
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7 Customer Q&As for Anti-BCAS3 Antibody
Question
Is a blocking peptide available for product anti-BCAS3 antibody (A07120)?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
We do provide the blocking peptide for product anti-BCAS3 antibody (A07120). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-10
Question
We are currently using anti-BCAS3 antibody A07120 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
The anti-BCAS3 antibody (A07120) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-06
Question
My question regarding product A07120, anti-BCAS3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07120 anti-BCAS3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-08
Question
Is this A07120 anti-BCAS3 antibody reactive to the isotypes of BCAS3?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
The immunogen of A07120 anti-BCAS3 antibody is Synthesized peptide derived from the Internal region of human BCAS3. at AA rangle: 660-740. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-25
Question
I see that the anti-BCAS3 antibody A07120 works with WB, what is the protocol used to produce the result images on the product page?
K. Anderson
Verified customer
Asked: 2017-08-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-08-22
Question
Does A07120 anti-BCAS3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Rodriguez
Verified customer
Asked: 2015-08-04
Answer
As indicated on the product datasheet, A07120 anti-BCAS3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-08-04
Question
I have attached the WB image, lot number and protocol we used for leukemic t-cell using anti-BCAS3 antibody A07120. Please let me know if you require anything else.
Z. Dhar
Verified customer
Asked: 2014-01-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-23
