Anti-Bcl-10 Antibody

Product Name

Anti-Bcl-10 Antibody

View all BCL10 Antibodies

SKU/Catalog Number

A01616

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Bcl-10 Antibody (Catalog # A01616). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Bcl-10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Bcl-10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01616)

Host

Rabbit

Contents

Bcl-10 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Bcl10 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Bcl10. The immunogen is located within the first 50 amino acids of Bcl-10.

Reactive Species

A01616 is reactive to BCL10 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

26.3 kDa

Background of BCL10

Apoptosis is related to many diseases including cancer. Cell death signals are transduced by death domain (DD) and caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include ARC, RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD containing protein was recently identified by several groups and designated Bcl10, CIPER, mE10, CARMEN, CLAP. Bcl10 is a cellular homolog of the equine herpesvirus-2 E-10 gene. Overexpression of Bcl10 induces JNK, p38, and NF-κB activation. Bcl10 interacts with caspase-9 and enhances pro-caspase-9 processing and induces apoptosis through caspase-9 activation. Bcl10 exhibits a variety of mutations in MALT lymphomas and in B and T cell lineage lymphomas indicating that it may be commonly involved in the pathogenesis of human malignancy. Bcl10 is expressed in many human and murine tissues and cell lines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A01616 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Bcl-10 antibody can be used for detection of BCL10 by Western blot at 0.5 μg/mL. dilution. An approximately 31 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BCL10

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis