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Product Info Summary
| SKU: | M04057 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-BCL9 Antibody (N-Term)
SKU/Catalog Number
M04057
Size
80 µl
Description
Boster Bio Anti-BCL9 Antibody (N-Term) (Catalog # M04057). Tested in WB, IF application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BCL9 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04057)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This BCL9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-304 amino acids from human BCL9.
Reactive Species
M04057 is reactive to BCL9 in Human, Mouse
Calculated molecular weight
149.3 kDa
Background of BCL9
Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04057 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling BCL9 with M04057 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
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All lanes : Anti-BCL9 Antibody (N-Term) at 1:2000 dilution
Lane 1: mouse spleen lysates
Lane 2: human testis lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 149 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-BCL9 Antibody (N-Term) (M04057)
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