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Product Info Summary
| SKU: | A04100-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-BCLAF1 Antibody
SKU/Catalog Number
A04100-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BCLAF1 Antibody (Catalog # A04100-1). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BCLAF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-BCLAF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04100-1)
Host
Rabbit
Contents
BCLAF1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BCLAF1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human BCLAF1. The immunogen is located within amino acids 820 - 870 of BCLAF1.
Cross-reactivity
BCLAF1 antibody is human, mouse and rat reactive. At least four isoforms of BCLAF1 are known to exist; this antibody will detect all but isoform 2.
Reactive Species
A04100-1 is reactive to BCLAF1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
106.1 kDa
Background of BCLAF1
The BCL2-associated transcription factor 1 (BCLAF1) is a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (1). BCLAF1 localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. (2). More recent studies have suggested BCLAF1 plays roles in processes not associated with actions of the BCL2 family members, including lung development, T cell activation, and post-transcriptional processes that affect mRNA metabolism (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04100-1 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BCLAF1 antibody can be used for detection of BCLAF1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BCLAF1 in human brain tissue lysate with BCLAF1 antibody at 1 μg/ml.
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Immunohistochemistry of BCLAF1 in mouse brain tissue with BCLAF1 antibody at 5 μg/mL.
Specific Publications For Anti-BCLAF1 Antibody (A04100-1)
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