Product Info Summary
| SKU: | M00035-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BDNF Rabbit Monoclonal Antibody
SKU/Catalog Number
M00035-2
BM4416 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-BDNF Rabbit Monoclonal Antibody catalog # M00035-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BDNF Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00035-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
17B21
Isotype
IgG
Immunogen
A synthesized peptide derived from human BDNF
Reactive Species
M00035-2 is reactive to BDNF in Human, Mouse, Rat
Observed Molecular Weight
28 kDa
Calculated molecular weight
27.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00035-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: Human breast cancer tissue, Human Hodgkin's lymphoma tissue, Human prostate cancer tissue, human brain tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
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Western blot analysis of extracts of Mouse heart lysate, using BDNF antibody
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Immunohistochemical analysis of paraffin-embedded Human breast cancer, using the Antibody at 1:1500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:1500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:1500 dilution.
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Immunohistochemical analysis of paraffin-embedded human brain, using BDNF Antibody.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
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Immunofluorescent analysis of Hela cells, using BDNF Antibody.
Specific Publications For Anti-BDNF Rabbit Monoclonal Antibody (M00035-2)
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