This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
BECN1 antibody (A00327-1) performs well in ELISA, IF, IHC-P, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
Filter:
Immunofluorescence Validation of Beclin-1 in HeLa Cells
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Beclin-1 with A00327-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Beclin-1 with A00327-1 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Designing a Western blot for BECN1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the BECN1 Western Blot GuideBundle Save
Anti-Beclin-1 BECN1 Antibody
BECN1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, ICC, WB |
Recommend Dilution
Application
Dilution
Species
WB: 1-2 μg/mL
IHC-P: 2-5 μg/mL
ICC/IF: 10-20 μg/mL
IHC-P/IF: 10-20 μg/mL.
Antibody validated: Western Blot in human
mouse and rat samples; Immunohistochemistry in human
mouse, and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Beclin-1 BECN1 Antibody
View all BECN1 Antibodies
View all BECN1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Beclin-1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
Beclin-1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Beclin-1 antibody was raised against a peptide corresponding to 17 amino acids near the center of human Beclin-1. The immunogen is located between 40-90 amino acids of Beclin-1.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
51.9 kDa
Background of BECN1
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.
Cite This Product
Anti-Beclin-1 BECN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00327-1)
Publications
Application:
Species:
Ni K,Hua Y.Hydrogen sulfide exacerbated periodontal inflammation and induced autophagy in experimental periodontitis.Int Immunopharmacol.2021 Jan 30;93:107399.doi:10.1016/j.intimp.2021.107399.Epub ahead of print.PMID:33529908.
Species: Human,Rat
PMID: 33529908
Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
PMID: 31900522
Review
Have you used Anti-Beclin-1 BECN1 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Beclin-1 BECN1 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A00327-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 27, 2026