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Product Info Summary
| SKU: | A01434-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Bestrophin-1/Best1 Antibody Picoband®
SKU/Catalog Number
A01434-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bestrophin-1/Best1 Antibody Picoband® catalog # A01936-4. Tested in WB, ELISA applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Bestrophin-1/Best1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01434-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived mouse Bestrophin-1/Best1 recombinant protein (Position: V9-E551). Mouse Best1 shares 64.3% amino acid (aa) sequence identity with human Best1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01434-2 is reactive to Best1 in Mouse, Rat
Observed Molecular Weight
56,60 kDa
Calculated molecular weight
63.7 kDa
Background of Best1
Bestrophin-1 (Best1) is a protein that, in humans, is encoded by the BEST1 gene. This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01434-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, rat C6 whole cell, mouse eye tissue, mouse brain tissue, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Western blot analysis of Bestrophin-1/Best1 Antibody using anti-Bestrophin-1/Best1 Antibody antibody (A01434-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse eye tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bestrophin-1/Best1 Antibody antigen affinity purified polyclonal antibody (Catalog # A01434-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bestrophin-1/Best1 Antibody at approximately 56,60 kDa. The expected band size for Bestrophin-1/Best1 Antibody is at 64 kDa.
Specific Publications For Anti-Bestrophin-1/Best1 Antibody Picoband® (A01434-2)
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