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Product Info Summary
| SKU: | A09370 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-beta-1,4-Gal-T5 B4GALT5 Antibody
SKU/Catalog Number
A09370
Size
100μl
Form
Liquid
Description
Boster Bio Anti-beta-1,4-Gal-T5 B4GALT5 Antibody catalog # A09370. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta-1,4-Gal-T5 B4GALT5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09370)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human B4GALT5. AA range:321-370
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A09370 is reactive to B4GALT5 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
45.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09370 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using β-1, 4-Gal-T5 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
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Western Blot analysis of 293 cells using β-1, 4-Gal-T5 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-beta-1,4-Gal-T5 B4GALT5 Antibody (A09370)
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Customer Q&As
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5 Customer Q&As for Anti-beta-1,4-Gal-T5 B4GALT5 Antibody
Question
I was wanting to use your anti- beta-1,4-Gal-T5 antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
You can see on the product datasheet, A09370 anti- beta-1,4-Gal-T5 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-07
Question
I have a question about product A09370, anti- beta-1,4-Gal-T5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Anderson
Verified customer
Asked: 2019-08-21
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09370 anti- beta-1,4-Gal-T5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-21
Question
Does anti- beta-1,4-Gal-T5 antibody A09370 work for WB with brain?
D. Dhar
Verified customer
Asked: 2019-08-19
Answer
According to the expression profile of brain, B4GALT5 is highly expressed in brain. So, it is likely that anti- beta-1,4-Gal-T5 antibody A09370 will work for WB with brain.
Boster Scientific Support
Answered: 2019-08-19
Question
We are currently using anti- beta-1,4-Gal-T5 antibody A09370 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
The anti- beta-1,4-Gal-T5 antibody (A09370) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-12
Question
Is there a BSA free version of anti- beta-1,4-Gal-T5 antibody A09370 available?
P. Patel
Verified customer
Asked: 2014-12-11
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti- beta-1,4-Gal-T5 antibody A09370 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-12-11
