Anti-beta-Actin ACTB Antibody (HRP)

beta-Actin antibody

Boster Bio Anti-beta-Actin ACTB Antibody (HRP) (Catalog # A01263-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. Cited in 10 publication(s).

Product Info Summary

SKU: A01263-HRP
Size: 100μg/vial
Reactive Species: Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Host: Rabbit
Application: WB

Product Name

Anti-beta-Actin ACTB Antibody (HRP)

View all beta-Actin Antibodies

SKU/Catalog Number

A01263-HRP

Size

100μg/vial

Form

Liquid

Description

Boster Bio Anti-beta-Actin ACTB Antibody (HRP) (Catalog # A01263-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish.

Storage & Handling

HRP-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-beta-Actin ACTB Antibody (HRP) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263-HRP)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.

Clonality

Polyclonal

Isotype

IgG

Immunogen

HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

HRP-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.

Reactive Species

A01263-HRP is reactive to ACTB in Chicken, Human, Monkey, Mouse, Rat, Zebrafish

Applications

A01263-HRP is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

41.737kDa

Background of beta-Actin

Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.02-0.1 μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)

Gene Name

ACTB

Full Name

Actin, cytoplasmic 1

Weight

41.737kDa

Superfamily

actin family

Alternative Names

ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTB, check out the ACTB Infographic

ACTB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01263-HRP has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu ZZ,Han ZD,Liang YK,Chen JX,Wan S,Zhuo YJ,Cai ZD,Deng YL,Lin ZY,Mo RJ,He HC,Zhong WD.TRIB1 induces macrophages to M2 phenotype by inhibiting IKB-zeta in prostate cancer.Cell Signal.2019 Jul;59:152-162.doi:10.1016/j.cellsig.2019.03.017.Epub 2019 Mar 26.PMID:30926388.
Species: Human,Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:PC3 CELL,DU145 CELL AND THP-1 CELL, DILUTION:1:3000

Xu Y,Wang P,Xu C,Shan X,Feng Q.Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression.J Biomed Res.2019 Jun 4;33(3):181-191.doi:10.7555/JBR.31.20170016.PMID:28963442;PMCID:PMC6551424.
Species: Human
A01263-HRP usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:NA

Guo J,Tu J,Hu Y,Song G,Yin Z.Cathepsin G cleaves and activates IL-36γ and promotes the inflammation of psoriasis.Drug Des Devel Ther.2019 Feb 8;13:581-588.doi:10.2147/DDDT.S194765.PMID:30804664;PMCID:PMC6372007.
Species: Human,Mouse

Chen B,Li X,Sun Y,Hou Y,Shen J,Tong C,Ma J.Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking.Exp Eye Res.2021 Jul 3:108695.doi:10.1016/j.exer.2021.108695.Epub ahead of print.PMID:34228966.
Species: New Zealand White Rabbit
A01263-HRP usage in article: APP:WB, SAMPLE: FIBROBLASTS, DILUTION:1:100

Cui J,Xu H,Yu J,Li Y,Chen Z,Zou Y,Zhang X,Du Y,Xia J,Wu J.IL-4 inhibits regulatory T cells differentiation by HDAC9-mediated epigenetic regulation.Cell Death Dis.2021 May 18;12(6):501.doi:10.1038/s41419-021-03769-7.PMID:34006836.
Species: Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:T CELL, DILUTION:1:1000

Huang X,Gan H,Tan J,Wang T,Zhao J,Zhao Y.BRCC3 promotes activation of the NLRP6 inflammasome following cerebral ischemia/reperfusion (I/R) injury in rats.Neurosci Lett.2021 May 9;756:135954.doi:10.1016/j.neulet.2021.135954.Epub ahead of print.PMID:33979701.
Species: Rat
A01263-HRP usage in article: APP:WB, SAMPLE:CEREBERAL CORTEX, DILUTION:1:2000

He G,Gu J,Wang H,Cheng S,Xiong Q,Ke M,Hu Y,Feng J,Song L,Liu Z,Xu Y.Nr2e1 deficiency aggravates insulin resistance and chronic inflammation of visceral adipose tissues in a diet-induced obese mice model.Life Sci.2021 Apr 26;278:119562.doi:10.1016/j.lfs.2021.119562.Epub ahead of print.PMID:33915130.
Species: Mouse
A01263-HRP usage in article: APP: WB, SAMPLE:ADIPOSE TISSUE, DILUTION:1:1000

Huaqing Zheng,Xiaolong Li,Xin Zeng,Chengcui Huang,Mingming Ma,Xiaofei Lv,Yajuan Zhang,Lu Sun,Guanlei Wang,Yanhua Du,Yongyuan Guan, TMEM16A inhibits angiotensin II-induced basilar artery smooth muscle cell migration in a WNK1-dependent manner,Acta Pharmaceutica Sinica B,2021,,ISSN 2211-3835,https://doi.org/10.1016/j.apsb.2021.04. 013.
Species: Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:BASMCS, DILUTION:NA

Hu J,Cai D,Zhao Z,Zhong G-C and Gong J (2021) Suppression of Heterogeneous Nuclear Ribonucleoprotein C Inhibit Hepatocellular Carcinoma Proliferation, Migration, and Invasion via Ras/MAPK Signaling Pathway.Front.Oncol.11:659676.doi:10.3389/fonc.2021.659676
Species: Human,Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:HCC TISSUE, DILUTION:1:2000

Lamptey J,Li F,Adu-Gyamfi EA,Chen XM,Czika A,Otoo A,Liu TH,Wang YX,Ding YB.Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.Exp Cell Res.2021 Apr 16;403(1):112597.doi:10.1016/j.yexcr.2021.112597.Epub ahead of print.PMID:33862100.
Species: Human
A01263-HRP usage in article: APP: WB, SAMPLE:PLACENTAL TISSUE, DILUTION: 1:1000

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Size

Total: $570

SKU:A01263-HRP

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Lead time for this item is typically 2-3 weeks

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A01263-HRP
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$570.00

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