Click image to see more details
Product Info Summary
| SKU: | A01263-HRP |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Chicken, Human, Monkey, Mouse, Rat, Zebrafish |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-beta-Actin ACTB Antibody Picoband® (HRP)
SKU/Catalog Number
A01263-HRP
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-beta-Actin ACTB Antibody (HRP) (Catalog # A01263-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
HRP-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-beta-Actin ACTB Antibody Picoband® (HRP) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263-HRP)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.
Cross-reactivity
HRP-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.
Reactive Species
A01263-HRP is reactive to ACTB in Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Observed Molecular Weight
42 kDa
Calculated molecular weight
41.7 kDa
Background of ACTB
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01263-HRP is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.02-0.1 μg/ml
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, human K562 whole cell, human A549 whole cell, human A431 whole cell, human U87 whole cell, monkey COS-7 whole cell, rat heart tissue, rat skeletal muscle tissue, rat brain tissue, rat PC-12 whole cell, mouse heart tissue, mouse skeletal tissue, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of beta-Actin using anti-beta-Actin antibody (A01263-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human U87 whole cell lysates,
Lane 8: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta-Actin antigen affinity purified polyclonal antibody (Catalog # A01263-HRP) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta-Actin at approximately 42 kDa. The expected band size for beta-Actin is at 42 kDa.
Click image to see more details
Western blot analysis of beta-Actin using anti-beta-Actin antibody (A01263-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta-Actin antigen affinity purified polyclonal antibody (Catalog # A01263-HRP) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta-Actin at approximately 42 kDa. The expected band size for beta-Actin is at 42 kDa.
Specific Publications For Anti-beta-Actin ACTB Antibody Picoband® (HRP) (A01263-HRP)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-beta-Actin ACTB Antibody Picoband® (HRP)?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-beta-Actin ACTB Antibody Picoband® (HRP)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
