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Product Info Summary
| SKU: | PA1872 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Chicken, Human, Monkey, Mouse, Rat, Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-beta Actin/ACTB Antibody Picoband®
SKU/Catalog Number
PA1872
BA2305 is an alternative SKU for this antibody, used in previous lots.
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-beta Actin/ACTB Antibody catalog # PA1872. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Actin/ACTB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1872)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
PA1872 is reactive to ACTB in Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Observed Molecular Weight
42 kDa
Calculated molecular weight
41.7 kDa
Background of ACTB
beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1872 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
Positive Control
WB: human Hela whole cell, human K562 whole cell, human MCF-7 whole cell, human Jurkat whole cell, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse kidney tissue, zebrafish tissue, chicken heart tissue, chicken liver tissue, chicken brain tissue, monkey heart tissue, monkey lung tissue, monkey kidney tissue, monkey liver tissue
Validation Images & Assay Conditions
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Western blot analysis of beta Actin/ACTB using anti-beta Actin/ACTB antibody (PA1872).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Actin/ACTB antigen affinity purified polyclonal antibody (Catalog # PA1872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Actin/ACTB at approximately 42 kDa. The expected band size for beta Actin/ACTB is at 42 kDa.
Click image to see more details
Western blot analysis of beta Actin/ACTB using anti-beta Actin/ACTB antibody (PA1872).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish tissue lysates,
Lane 2: chicken heart tissue lysates,
Lane 3: chicken liver tissue lysates,
Lane 4: chicken brain tissue lysates,
Lane 5: monkey heart tissue lysates,
Lane 6: monkey lung tissue lysates,
Lane 7: monkey kidney tissue lysates,
Lane 8: monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta Actin/ACTB antigen affinity purified polyclonal antibody (Catalog # PA1872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta Actin/ACTB at approximately 42 kDa. The expected band size for beta Actin/ACTB is at 42 kDa.
Specific Publications For Anti-beta Actin/ACTB Antibody Picoband® (PA1872)
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