Anti-beta Actin/ACTB Antibody

beta-Actin antibody

Boster Bio Anti-beta Actin/ACTB Antibody catalog # PA1872. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish. Cited in 10 publication(s).

Product Info Summary

SKU: PA1872
Size: 100μg/vial
Reactive Species: Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Host: Rabbit
Application: WB

Product Name

Anti-beta Actin/ACTB Antibody

View all beta-Actin Antibodies

SKU/Catalog Number

PA1872

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-beta Actin/ACTB Antibody catalog # PA1872. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-beta Actin/ACTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1872)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins

Reactive Species

PA1872 is reactive to ACTB in Chicken, Human, Monkey, Mouse, Rat, Zebrafish

Applications

PA1872 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

41.737kDa

Background of beta-Actin

beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat, Chicken, Zebrafish

Validation Images & Assay Conditions

Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)

Gene Name

ACTB

Full Name

Actin, cytoplasmic 1

Weight

41.737kDa

Superfamily

actin family

Alternative Names

ACTB; actin, beta; actin, cytoplasmic 1; beta actin; B-Actin; beta actin (ac-15); Beta Actin; beta cytoskeletal actin; beta-actin ac 15; beta-actin ac15; beta-actin ac-15; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1 ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ACTB, check out the ACTB Infographic

ACTB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1872 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The natural product mensacarcin induces mitochondrial toxicity and apoptosis in melanoma cells

Yang X,Liu H,Ye T,Duan C,Lv P,Wu X,Liu J,Jiang K,Lu H,Yang H,Xia D,Peng E,Chen Z,Tang K,Ye Z. AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization. Theranostics.2020
Species: Mouse

Zhai L, Liu M, Wang T, Zhang H, Li S, Guo Y. PLoS One. 2017 Apr 7;12(4):e0174414. doi: 10.1371/journal.pone.0174414. eCollection 2017. Picroside II protects the blood-brain barrier by inhibiting the oxidative signaling pathway in cerebral ischemia...

Xiaodan Chang, Baohong Zhang, Li Lihua, Zhichun Feng Am J Transl Res. 2016 Nov 15;8(11):4694-4704. eCollection 2016. T3 inhibits the calcification of vascular smooth muscle cells and the potential mechanism.

Du B, Li L, Zhong Z, Fan X, Qiao B, He C, Fu Z, Wang Y, Ye Q. Int J Mol Med. 2014 Aug;34(2):578-84. Doi: 10.3892/Ijmm.2014.1806. Epub 2014 Jun 16. Brain Death Induces The Alteration Of Liver Protein Expression Profiles In Rabbits.

Wang Wq, Zhang H, Wang Hb, Sun Yg, Peng Zh, Zhou G, Yang Sm, Wang Rq, Fang Dc. Mol Diagn Ther. 2010 Jun 1;14(3):155-61. Doi: 10.2165/11536340-000000000-00000. Programmed Cell Death 4 (Pdcd4) Enhances The Sensitivity Of Gastric Cancer Cells To Trai...

Wang H, Qi J, Li L, Wu T, Wang Y, Wang X, Ning Q. Int J Immunopathol Pharmacol. 2015 Sep;28(3):308-17. Doi: 10.1177/0394632015589519. Epub 2015 Jul 8. Inhibitory Effects Of Chikusetsusaponin Iva On Lipopolysaccharide-Induced Pro-Inflammatory Respo...

Wang J, Zhao Q, Qi Q, Gu Hy, Rong Jj, Mu R, Zou Mj, Tao L, You Qd, Guo Ql. J Cell Biochem. 2011 Feb;112(2):509-19. Doi: 10.1002/Jcb.22941. Gambogic Acid-Induced Degradation Of Mutant P53 Is Mediated By Proteasome And Related To Chip.

Zheng Z, Ding M, Ni J, Song D, Huang J, Wang J. Oncol Rep. 2015 Mar;33(3):1291-9. Doi: 10.3892/Or.2014.3687. Epub 2014 Dec 22. Mir-142 Acts As A Tumor Suppressor In Osteosarcoma Cell Lines By Targeting Rac1.

Bi Y, Sun Wp, Chen X, Li M, Liang H, Cai My, Zhu Yh, He Xy, Xu F, Weng Jp. Acta Diabetol. 2008 Sep;45(3):167-78. Doi: 10.1007/S00592-008-0038-7. Epub 2008 May 24. Effect Of Early Insulin Therapy On Nuclear Factor Kappab And Cytokine Gene Expressio...

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Size

Conjugation

Total: $370

SKU:PA1872

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Lead time for this item is typically 2-3 weeks

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PA1872
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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