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- Table of Contents
CTNNB1 antibody (M00004-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of SiHa cells using anti-beta Catenin antibody (M00004-2).
Overlay histogram showing SiHa cells stained with M00004-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-beta Catenin Antibody (M00004-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing SiHa cells stained with M00004-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-beta Catenin Antibody (M00004-2,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6)
CTNNB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CTNNB1 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, human A431 whole cell, human RT4 whole cell, human T-47D whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CTNNB1 antibody was positively detected by IHC in:
human mammary cancer, human mammary cancer
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CTNNB1 antibody was positively detected by ICC/IF in:
A431 cell
CTNNB1 antibody was positively detected by ICC in:
A549 Cell
CTNNB1 antibody was positively detected by FC in:
SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6)
View all CTNNB1 Antibodies
View all CTNNB1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
1F6
Isotype
Mouse IgG1
Immunogen
E. coli-derived human beta Catenin recombinant protein (Position: A2-K233).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
85.5 kDa
Background of CTNNB1
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Cite This Product
Anti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00004-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Monkey
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Zebrafish
95%
High Homology
Drosophila
66%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CTNNB1 antibody
Immunohistochemistry (IHC) protocol for CTNNB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CTNNB1 antibody
Immunocytochemistry (ICC) protocol for CTNNB1 antibody
Flow Cytometry (FC) protocol for CTNNB1 antibody
Publications
Application:
Species:
Combined miR-181a-5p and Ag Nanoparticles are Effective Against Oral Cancer in a Mouse Model
Published: 2024-09-06
Journal: International Journal of Nanomedicine
Application: WB
Impact Factor: 6.60
Species: Human
PMID: 39267724
VEGF overexpression in transplanted NSCs promote recovery of neurological function in rats with cerebral ischemia by modulating the Wnt signal transduction pathway
Published: 2024-02-07
Journal: NEUROSCIENCE LETTERS
Application: IHC
Impact Factor: 2.50
Species: Rat
PMID: 38331020
Up-regulation of E-cadherin by saRNA inhibits the migration and invasion of renal carcinoma cells
Published: 2018-12-01
Journal: International Journal of Clinical and Experimental Pathology
Application: WB
Impact Factor: 1.40
Species: Human
PMID: 31949665
Expression and clinical significance of HOXA5, E-cadherin, and β-catenin in cervical squamous cell carcinoma
Published: 2018-06-01
Journal: International Journal of Clinical and Experimental Pathology
Application: IHC
Impact Factor: 1.40
Species: Human
PMID: 31938436
Review
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Product Promise
We promise Anti-beta Catenin CTNNB1 Antibody Picoband® (monoclonal, 1F6) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00004-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026