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- Table of Contents
CTNNB1 antibody (M00004-1) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Immunofluorescent analysis of A431 cells, using beta Catenin Antibody .
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Anti-beta Catenin CTNNB1 Rabbit Monoclonal Antibody
CTNNB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IF, IHC, ICC, WB |
Tested Applications
CTNNB1 antibody was positively detected by WB in:
human HepG2 whole cell, human MCF-7 whole cell, rat PC-12 whole cell, rat RH35 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CTNNB1 antibody was positively detected by IHC in:
human bladder squamous cell carcinomar tissue, human liver cancer tissue, human ovarian cancer tissue, human placenta tissue, rat intestines tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CTNNB1 antibody was positively detected by ICC/IF in:
A431 cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-beta Catenin CTNNB1 Rabbit Monoclonal Antibody
View all CTNNB1 Antibodies
View all CTNNB1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta Catenin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated Molecular Weight
85.5 kDa
Cite This Product
Anti-beta Catenin CTNNB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00004-1)
Protocols
Western Blot protocol for CTNNB1 antibody
Immunohistochemistry (IHC) protocol for CTNNB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CTNNB1 antibody
Publications
Application:
Species:
Effects of bergapten-loaded long-circulating liposomes on osteogenic differentiation of human dental pulp stem cells
Published: 2025-10-29
Journal: JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
Application: WB,IF
Impact Factor: 1.30
Species: Human
PMID: 41160646
Sinapic acid and glabridin synergistically mitigate androgenetic alopecia by modulating ferroptosis through stabilization of β-catenin against ubiquitin-dependent degradation
Published: 2025-07-16
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 40680866
Di-(2-ethylhexyl) Phthalate (DEHP) and Its Metabolite Promote Multiple Myeloma Progression and Bortezomib Resistance
Published: 2025-07-04
Journal: JOURNAL OF HAZARDOUS MATERIALS
Application: WB,IF
Impact Factor: 11.30
Species: Mouse,Human
PMID: 40633344
CD168+ macrophages promote hepatocellular carcinoma tumor stemness and progression through TOP2A/β-catenin/YAP1 axis
Published: 2023-05-12
Journal: iScience
Application: Co-IP
Impact Factor: 5.80
Species: Human
PMID: 37275516
TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis
Published: 2022-01-14
Journal: Biology-Basel
Application: WB
Impact Factor: 5.17
Species: Human
PMID: 35053139
Downregulation of CXXC Finger Protein 4 Leads to a Tamoxifen-resistant Phenotype in Breast Cancer Cells Through Activation of the Wnt/β-catenin Pathway
Published: 2020-01-03
Journal: Translational Oncology
Application: IHC
Impact Factor: 3.56
Species: Mouse
PMID: 31911277
Sohlh1 suppresses glioblastoma cell proliferation, migration, and invasion by inhibition of Wnt/β-catenin signaling
Published: 2017-12-14
Journal: MOLECULAR CARCINOGENESIS
Application: WB
Impact Factor: 4.19
Species: Mouse
PMID: 29240260
Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells
Published: 2017-10-23
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.26
Species: Mouse
PMID: 29250149
E‑cadherin involvement in human lens epithelial cell transdifferentiation may be associated with N‑cadherin
Published: 2017-08-02
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.69
Species: Human
PMID: 28765930
Lithium chloride decreases proliferation and migration of C6 glioma cells harboring isocitrate dehydrogenase 2 mutant via GSK-3β
Published: 2014-02-19
Journal: MOLECULAR BIOLOGY REPORTS
Application: WB
Impact Factor: 1.96
Species: Rat
PMID: 24549719
Review
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Product Promise
We promise Anti-beta Catenin CTNNB1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M00004-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026