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- Table of Contents
TUBB1 antibody (M05397) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of TUBB1 using anti-TUBB1 antibody (M05397).
TUBB1 was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-TUBB1 Antibody (M05397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
TUBB1 was detected in a paraffin-embedded section of human clear cell renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-TUBB1 Antibody (M05397) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-beta I Tubulin TUBB1 Rabbit Monoclonal Antibody
TUBB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
TUBB1 antibody was positively detected by WB in:
human Hela whole cell, human PC-3 whole cell, human A549 whole cell, human MCF-7 whole cell, rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TUBB1 antibody was positively detected by IHC in:
human clear cell renal cell carcinoma tissue, human clear cell renal cell carcinoma tissue, human liver cancer tissue, human liver cancer tissue, human ovarian cancer tissue, human ovarian cancer tissue, human placenta tissue, human placenta tissue, human rectum adenocarcinoma tissue, human rectum adenocarcinoma tissue, human thyroid papillary carcinoma tissue, human thyroid papillary carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TUBB1 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:1000-5000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-beta I Tubulin TUBB1 Rabbit Monoclonal Antibody
View all TUBB1 Antibodies
View all TUBB1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CO-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta I Tubulin
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
50.3 kDa
Cite This Product
Anti-beta I Tubulin TUBB1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05397)
Protocols
Western Blot protocol for TUBB1 antibody
Immunohistochemistry (IHC) protocol for TUBB1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TUBB1 antibody
Publications
Application:
Species:
Subchronic benzo[a]pyrene exposure disrupts APOE4-regulated lipid metabolism to induce Tau hyperphosphorylation and cognitive deficits
Published: 2026-07-23
Journal: NEUROTOXICOLOGY
Application: WB
Impact Factor: 3.80
Species: Mouse
PMID: 42492781
Maternal Exposure of SD Rats to Benzo[a]Pyrene Impairs Neurobehavior and Hippocampal Synaptic Ultrastructure in Offspring via Downregulating Synaptic-Associated Factors
Published: 2025-02-18
Journal: ENVIRONMENTAL TOXICOLOGY
Application: WB
Impact Factor: 3.20
Species: Rat
PMID: 39967322
Celastrol-loaded biomimetic nanodrug ameliorates APAP-induced liver injury through modulating macrophage polarization
Published: 2023-05-02
Journal: JOURNAL OF MOLECULAR MEDICINE-JMM
Application: WB
Impact Factor: 4.70
Species: Mouse
PMID: 37129620
Prenatal LPS Exposure Promotes Allergic Airway Inflammation via Long Coding RNA NONMMUT033452.2, and Protein Binding Partner, Eef1D
Published: 2023-01-05
Journal: AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
Application: WB
Impact Factor: 6.40
Species: Mouse
PMID: 36603194
Prenatal benzo[a]pyrene exposure impairs hippocampal synaptic plasticity and cognitive function in SD rat offspring during adolescence and adulthood via HDAC2-mediated histone deacetylation
Published: 2022-10-17
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 7.13
Species: Rat
PMID: 36265406
Butyrate alleviates oxidative stress by regulating NRF2 nuclear accumulation and H3K9/14 acetylation via GPR109A in bovine mammary epithelial cells and mammary glands
Published: 2020-01-21
Journal: FREE RADICAL BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 6.17
Species: Cow
PMID: 31972340
Review
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Product Promise
We promise Anti-beta I Tubulin TUBB1 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M05397
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026