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Product Info Summary
| SKU: | M09008 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Tubulin beta 4B/2A/2B Rabbit Monoclonal Antibody
SKU/Catalog Number
M09008
BM4264 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Tubulin beta 4B/2A/2B Rabbit Monoclonal Antibody catalog # M09008. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tubulin beta 4B/2A/2B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09008)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
DOF-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Tubulin beta 2B
Reactive Species
M09008 is reactive to TUBB4B in Human, Mouse, Rat
Observed Molecular Weight
50 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09008 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Tubulin beta 4B/2A/2B using anti-Tubulin beta 4B/2A/2B antibody (M09008).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin beta 4B/2A/2B antigen affinity purified monoclonal antibody (Catalog # M09008) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin beta 4B/2A/2B at approximately 50 kDa. The expected band size for Tubulin beta 4B/2A/2B is at 50 kDa.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using beta II Tubulin Antibody.
Specific Publications For Anti-Tubulin beta 4B/2A/2B Rabbit Monoclonal Antibody (M09008)
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Customer Q&As
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7 Customer Q&As for Anti-Tubulin beta 4B/2A/2B Rabbit Monoclonal Antibody
Question
Would anti-beta II Tubulin Rabbit Monoclonal antibody M09008 work for WB with epithelium of bronchus?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
According to the expression profile of epithelium of bronchus, TUBB4B is highly expressed in epithelium of bronchus. So, it is likely that anti-beta II Tubulin Rabbit Monoclonal antibody M09008 will work for WB with epithelium of bronchus.
Boster Scientific Support
Answered: 2019-09-05
Question
Is there a BSA free version of anti-beta II Tubulin Rabbit Monoclonal antibody M09008 available?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-beta II Tubulin Rabbit Monoclonal antibody M09008 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-01
Question
Will M09008 anti-beta II Tubulin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
You can see on the product datasheet, M09008 anti-beta II Tubulin Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-29
Question
We are currently using anti-beta II Tubulin Rabbit Monoclonal antibody M09008 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
The anti-beta II Tubulin Rabbit Monoclonal antibody (M09008) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-08
Question
I see that the anti-beta II Tubulin Rabbit Monoclonal antibody M09008 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-10-02
Question
Here is the WB image, lot number and protocol we used for epithelium of bronchus using anti-beta II Tubulin Rabbit Monoclonal antibody M09008. Please let me know if you require anything else.
G. Jha
Verified customer
Asked: 2013-06-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-04
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for epithelium of bronchus using anti-beta II Tubulin Rabbit Monoclonal antibody M09008. Let me know if you need anything else.
S. Wu
Verified customer
Asked: 2013-01-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-07
