Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1)

Boster Bio Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) catalog # MA1112. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 18 publication(s).

Product Info Summary

SKU: MA1112
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: IF, IHC, ICC, WB

Product Name

Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1)

See all TUBB1 products

SKU/Catalog Number

MA1112

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) catalog # MA1112. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1112)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

TUB 2.1

Isotype

Mouse IgG1

Immunogen

Tubulin from rat brain.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1112 is reactive to TUBB1 in Human, Mouse, Rat

Applications

MA1112 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat
Western blot, 2μg/ml, Human, mouse, rat
Immunocytochemistry/Immunofluorescence,5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For TUBB1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9H4B7

Gene ID

81027

Gene Name

TUBB1

Full Name

Tubulin beta-1 chain

Weight

50.327kDa

Superfamily

tubulin family

Alternative Names

beta tubulin 1, class VI; betaI Tubulin; beta-I Tubulin; dJ543J19.4; TUBB1; tubulin beta-1 chain; tubulin, beta 1 TUBB1 tubulin beta 1 class VI tubulin beta-1 chain|tubulin, beta 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TUBB1, check out the TUBB1 Infographic

TUBB1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TUBB1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1112 has been cited in 18 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Han Zhang,Huaishan Wang,Fei Gao,Jia Yang,Yi Xu,Yi Fu,Menghua Cai,Xue Zhang,Qi Yang,Kexin Tong,Yu Hu,Hui Chen,Chao Ma,Wei He,Jianmin Zhang,TSPO Deficiency Accelerates Amyloid Pathology and Neuroinflammation by Impairing Microglial Phagocytosis,Neurobiology of Aging,2021,,ISSN 0197-4580,https://doi.org/10.1016/j.neurobiolaging.2021.06.020.
Species: Human,Mouse
MA1112 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Shi,W.,Que,Y.,Zhang,X.et al.Functional tissue-engineered bone-like graft made of a fibrin scaffold and TG2 gene-modified EMSCs for bone defect repair.NPG Asia Mater 13,28 (2021).https://doi.org/10.1038/s41427-021-00297-w
Species: Rat
MA1112 usage in article: APP:WB, SAMPLE:EMSCS, DILUTION:NA

Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973. doi:10.1155/2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse

Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973.doi:10.1155/ 2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse

Xu T,Song Q,Zhou L,Yang W,Wu X,Qian Q,Chai H,Han Q,Pan H,Dou X,Li S.Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes.Nutr Metab (Lond).2
Species: Mouse
MA1112 usage in article: APP:WB, SAMPLE:AML-12 CELL, DILUTION:NA

Xu T,Song Q,Zhou L,Yang W,Wu X,Qian Q,Chai H,Han Q,Pan H,Dou X,Li S. Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes. Nutr Metab (Lond)
Species: Mouse
MA1112 usage in article: APP:WB, SAMPLE:AML-12 CELLS, DILUTION:NA

Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
MA1112 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Fan J,Zhang YC,Zheng DF,Zhang M,Liu H,He M,Wu ZJ.IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model. Cytokine.2020 Mar;127:154936.doi: 10.1016/j.cyto.2019.154936.Epub 2019 Nov 28.PMID:317865
Species: Human, Mouse
MA1112 usage in article: APP:WB, SAMPLE:LIVER, DILUTION:1:1000

Propofol induces apoptosis and inhibits the proliferation of rat embryonic neural stem cells via gamma-aminobutyric acid type A receptor

Pim-3 is a Critical Risk Factor in Development and Prognosis of Prostate Cancer

Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats

Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats

Wang J, Guo XJ, Ding YM, Jiang JX. World J Gastroenterol. 2017 Mar 7;23(9):1594-1601. doi: 10.3748/wjg.v23.i9.1594. miR-1181 inhibits invasion and proliferation via STAT3 in pancreatic cancer

Wang, Y., Xu, C., Xu, Z., Ji, C., Liang, J., Wang, Y.,…, & Chen, Z. (2017). Depolarized GABAergic Signaling in Subicular Microcircuits Mediates Generalized Seizure in Temporal Lobe Epilepsy. Neuron, 95(1), 92-105.e5. doi: 10.1016/j.neuron.2017.06.004

Shang T, Liu L, Zhou J, Zhang M, Hu Q, Fang M, Wu Y, Yao P, Gong Z. Lipids Health Dis. 2017 Mar 29;16(1):65. doi: 10.1186/s12944-017-0461-2. Protective effects of various ratios of DHA/EPA supplementation on high-fat diet-induced liver damage in m...

Ye Y, Zeng Ym, Wan Mr, Lu Xf. Cell Biochem Biophys. 2011 Apr;59(3):179-84. Doi: 10.1007/S12013-010-9130-Z. Induction Of Human Bone Marrow Mesenchymal Stem Cells Differentiation Into Neural-Like Cells Using Cerebrospinal Fluid.

Chen Xh, Bao Yy, Zhou Sh, Wang Qy, Wei Y, Fan J. Mol Med Rep. 2013 Dec;8(6):1695-700. Doi: 10.3892/Mmr.2013.1740. Epub 2013 Oct 18. Glucose Transporter-1 Expression In Cd133+ Laryngeal Carcinoma Hep-2 Cells.

Zuo Q, Liu J, Zhang J, Wu M, Guo L, Liao W. Sci Rep. 2015 Jun 25;5:11634. Doi: 10.1038/Srep11634. Development Of Trastuzumab-Resistant Human Gastric Carcinoma Cell Lines And Mechanisms Of Drug Resistance.

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4 Customer Q&As for Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1)

Question

Will anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 work on dog IHC with brain?

Verified Customer

Verified customer

Asked: 2019-08-27

Answer

Our lab technicians have not tested anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-27

Question

We are currently using anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?

E. Walker

Verified customer

Asked: 2018-10-17

Answer

The anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) (MA1112) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-10-17

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112. Let me know if you need anything else.

P. Mangal

Verified customer

Asked: 2018-08-06

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-08-06

Question

We are interested in to test anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 on human platelet for research purposes, then I may be interested in using anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Anderson

Verified customer

Asked: 2017-05-12

Answer

The products we sell, including anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-05-12

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