radio
SKU MA1112
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number TUB 2.1
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-Beta-Tubulin Antibody (Monoclonal, TUB 2.1)
SKU/Catalog Number MA1112
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for beta-Tubulin detection. Tested with WB, IHC-P in Human,mouse,rat. No cross reactivity with other proteins.
Cite This Product Anti-Beta-Tubulin Antibody (Monoclonal, TUB 2.1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1112)
Host Mouse
Immunogen Tubulin from rat brain.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat
Western blot, 2μg/ml, Human, mouse, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).

Images And Assay Conditions

/antibody/ma1112 1 WB anti beta tubulin antibody monoclonal tub 2 1.jpg

Anti-beta-Tubulin antibody (monoclonal), MA1112, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate

/antibody/ma1112 2 IHC anti beta tubulin antibody monoclonal tub 2 1.jpg

Anti-beta-Tubulin antibody (monoclonal), MA1112, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q9H4B7
Gene Name TUBB1
Protein Name Tubulin beta-1 chain
Tissue Specificity Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins. .
Alternative Names Tubulin beta-1 chain;TUBB1;
Subcellular Localization Cytoplasm, cytoskeleton .
Molecular Weight 50327 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). .
Background Tubulin beta(TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.

Order Product (MA1112)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Guaranteed product quality

We promise all of our products perform as described in datasheets.

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Publications

Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Shang T, Liu L, Zhou J, Zhang M, Hu Q, Fang M, Wu Y, Yao P, Gong Z. Lipids Health Dis. 2017 Mar 29;16(1):65. doi: 10.1186/s12944-017-0461-2. Protective effects of various ratios of DHA/EPA supplementation on high-fat diet-induced liver damage in mice
Wang, Y., Xu, C., Xu, Z., Ji, C., Liang, J., Wang, Y.,…, & Chen, Z. (2017). Depolarized GABAergic Signaling in Subicular Microcircuits Mediates Generalized Seizure in Temporal Lobe Epilepsy. Neuron, 95(1), 92-105.e5. doi: 10.1016/j.neuron.2017.06.004
Ye Y, Zeng Ym, Wan Mr, Lu Xf. Cell Biochem Biophys. 2011 Apr;59(3):179-84. Doi: 10.1007/S12013-010-9130-Z. Induction Of Human Bone Marrow Mesenchymal Stem Cells Differentiation Into Neural-Like Cells Using Cerebrospinal Fluid.
Chen Xh, Bao Yy, Zhou Sh, Wang Qy, Wei Y, Fan J. Mol Med Rep. 2013 Dec;8(6):1695-700. Doi: 10.3892/Mmr.2013.1740. Epub 2013 Oct 18. Glucose Transporter-1 Expression In Cd133+ Laryngeal Carcinoma Hep-2 Cells.
Zuo Q, Liu J, Zhang J, Wu M, Guo L, Liao W. Sci Rep. 2015 Jun 25;5:11634. Doi: 10.1038/Srep11634. Development Of Trastuzumab-Resistant Human Gastric Carcinoma Cell Lines And Mechanisms Of Drug Resistance.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to tubb antibody, tubb2a antibody