Anti-Beta Tubulin Antibody Picoband™(monoclonal, 5E4)

beta Tubulin antibody

Boster Bio Anti-Beta Tubulin Antibody Picoband™ (monoclonal, 5E4) catalog # M01857-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: M01857-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-Beta Tubulin Antibody Picoband™(monoclonal, 5E4)

View all beta Tubulin Antibodies

SKU/Catalog Number

M01857-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Beta Tubulin Antibody Picoband™ (monoclonal, 5E4) catalog # M01857-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beta Tubulin Antibody Picoband™(monoclonal, 5E4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01857-3)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

5E4

Isotype

Mouse IgG1

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01857-3 is reactive to TUBB in Human, Mouse, Rat

Applications

M01857-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

49.671kDa

Background of beta Tubulin

Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For TUBB (Source: Uniprot.org, NCBI)

Gene Name

TUBB

Full Name

Tubulin beta chain

Weight

49.671kDa

Superfamily

tubulin family

Alternative Names

beta polypeptide; MGC117247; Tubb5; tubulin beta chain; tubulin beta polypeptide; tubulin beta-1 chain; Tubulin beta-5 chain; tubulin, beta TUBB CDCBM6, CSCSC1, M40, OK/SW-cl.561, TUBB5, TUBB tubulin beta class I tubulin beta chain|beta Ib tubulin|epididymis secretory sperm binding protein|tubulin beta-1 chain|tubulin beta-5 chain|tubulin, beta polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TUBB, check out the TUBB Infographic

TUBB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TUBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01857-3 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Shen X,Zhang J,Zhang X,Wang Y,Hu Y,Guo J.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.Cell Mol Neurobiol.2019 Mar;39(2):241-254.doi:10.1007/s10571-018-0644-z.Epub 2018 Dec 15.PMID:30554401.
Species: Human
M01857-3 usage in article: APP:WB, SAMPLE:HUVECS, DILUTION:1:300

Han Zhang,Huaishan Wang,Fei Gao,Jia Yang,Yi Xu,Yi Fu,Menghua Cai,Xue Zhang,Qi Yang,Kexin Tong,Yu Hu,Hui Chen,Chao Ma,Wei He,Jianmin Zhang,TSPO Deficiency Accelerates Amyloid Pathology and Neuroinflammation by Impairing Microglial Phagocytosis,Neurobiology of Aging,2021,,ISSN 0197-4580,https://doi.org/10.1016/j.neurobiolaging.2021.06.020.
Species: Human,Mouse
M01857-3 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA

Yu Jiao,Ran Hu,Sirong Hu,Binge Wang,Dehong Huang,Zhou Lan,Shenrong Wuzi Pill affects the pathway of thyroid hormone synthesis by targeting thyrotropin-releasing hormone receptor and altering cAMP production,Journal of Herbal Medicine,2021,100470,ISSN 2210-8033,https://doi.org/10.1016/j. hermed. 2021.100470.
Species: Mouse
M01857-3 usage in article: APP:WB, SAMPLE:NG108-15 CELL, DILUTION: 1:3000

Shi,W.,Que,Y.,Zhang,X.et al.Functional tissue-engineered bone-like graft made of a fibrin scaffold and TG2 gene-modified EMSCs for bone defect repair.NPG Asia Mater 13,28 (2021).https://doi.org/10.1038/s41427-021-00297-w
Species: Rat
M01857-3 usage in article: APP:WB, SAMPLE:EMSCS, DILUTION:NA

Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973. doi:10.1155/2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
M01857-3 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000

Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973.doi:10.1155/ 2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
M01857-3 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000

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Order DetailsPrice
M01857-3

100μg

$370
M01857-3-10ug

10μg sample (liquid)

$99
M01857-3-Biotin

100 μg Biotin conjugated

$570
M01857-3-Cy3

100 μg Cy3 conjugated

$570
M01857-3-Dylight488

100 μg Dylight488 conjugated

$570
M01857-3-Dylight550

100 μg Dylight550 conjugated

$570
M01857-3-Dylight594

100 μg Dylight594 conjugated

$570
M01857-3-FITC

100 μg FITC conjugated

$570
M01857-3-HRP

100 μg HRP conjugated

$570
M01857-3-APC

100 μg APC conjugated

$670
M01857-3-PE

100 μg PE conjugated

$670
M01857-3-carrier-free

Carrier Free

$370

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M01857-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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