Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated

Product Name

Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated

View all beta-III Tubulin Antibodies

SKU/Catalog Number

H01857

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated catalog # H01857. Tested in WB application. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-beta Tubulin Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # H01857)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DF-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human beta Tubulin

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

H01857 is reactive to TUBB3 in Human, Mouse, Rat

Applications

H01857 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

50.433kDa

Background of beta-III Tubulin

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:5000-1:20000

Validation Images & Assay Conditions

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Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate (H01857).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB3 monoclonal antibody (Catalog # H01857) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBB3

Gene/Protein Information For TUBB3 (Source: Uniprot.org, NCBI)

Alternative Names

Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen TUBB3 CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4 tubulin beta 3 class III tubulin beta-3 chain|class III beta-tubulin|tubulin beta-4 chain|tubulin beta-III|tubulin, beta 3

For more info on TUBB3, check out the TUBB3 Infographic

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In this infographic, you will see the following information for TUBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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