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Product Info Summary
| SKU: | A10479-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BFAR Antibody
SKU/Catalog Number
A10479-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BFAR Antibody (Catalog # A10479-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BFAR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BFAR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10479-1)
Host
Rabbit
Contents
BFAR Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BFAR antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BFAR. The immunogen is located within amino acids 300 - 350 of BFAR.
Reactive Species
A10479-1 is reactive to BFAR in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.7 kDa
Background of BFAR
The bifunctional apoptosis inhibitor (BFAR) is scaffold protein that integrates signaling components of the cells apoptosis-regulatory machinery. BFAR is a multidomain protein capable of inhibiting apoptosis induced by TNF-family death receptors ('extrinsic pathway') as well as mitochondria-dependent apoptosis ('intrinsic pathway'). Interaction of BFAR with Bcl-2 or Bcl-XL via a SAM domain may contribute to the anti-apoptotic properties of BFAR. In addition, BFAR contains a DED-like domain that is capable of suppressing apoptosis mediated at the receptor level. BFAR is also thought to be involved in the regulation of neuronal survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10479-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BFAR antibody can be used for detection of BFAR by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BFAR in human kidney tissue lysate with BFAR antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of BFAR in mouse kidney tissue with BFAR antibody at 5 μg/mL.
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Immunofluorescence of BFAR in mouse kidney tissue with BFAR antibody at 20 μg/mL.
Specific Publications For Anti-BFAR Antibody (A10479-1)
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