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Product Info Summary
| SKU: | A10479 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-BFAR (BAR) Antibody
SKU/Catalog Number
A10479
Size
50 µl
Form
Liquid
Description
Boster Bio Anti-BFAR (BAR) Antibody (Catalog# A10479). Tested in IP, IHC, WB application(s). This antibody reacts with Human.
Storage & Handling
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Cite This Product
Anti-BFAR (BAR) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10479)
Host
Rabbit
Contents
50 µl sera
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide of BFAR (BAR) protein (amino acids 3-21 EPQKSYVNTMDLERDEPLK)
Reactive Species
A10479 is reactive to BFAR in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10479 is guaranteed for IP, IHC, WB Boster Guarantee
Recommend Dilution
WB: 1:1000-1:2000
IHC (paraffin): 1:1000-1:5000
IHC (frozen): Users should optimize
IP: 1:50-1:200
Validation Images & Assay Conditions
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Fig:1 Western blot analysis of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kDa. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
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Fig:2 Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using A10479 at 1:2000. Hematoxylin-eosin counterstain. Variable BAR expression is seen between patient samples
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Fig:3 Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using A10479 at 1:2000. A. Two cores from a human gliobastoma tissue microarray: 1 = fibrillary astrocytoma (grade I), and 2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-eosin counterstain.
Specific Publications For Anti-BFAR (BAR) Antibody (A10479)
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1 Customer Q&As for Anti-BFAR (BAR) Antibody
Question
We are currently using anti-BFAR (BAR) antibody A10479 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
The anti-BFAR (BAR) antibody (A10479) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-01
