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Product Info Summary
| SKU: | M04668 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-BHLH3 Antibody (N-term)
SKU/Catalog Number
M04668
Size
50 µl
Description
Boster Bio Anti-BHLH3 Antibody (N-term) (Catalog # M04668). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BHLH3 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04668)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
2135CT82.1.84
Isotype
IgG2b,κ
Immunogen
This BHLH3 antibody is generated from a mouse immunized with a recombinant protein of human BHLH3.
Reactive Species
M04668 is reactive to BHLHE41 in Human, Mouse, Rat
Calculated molecular weight
50.5 kDa
Background of BHLHE41
Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04668 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-BHLH3 Antibody (N-term) at 1:4000 dilution
Lane 1: HeLa whole cell lysate
Lane 2: RD whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: HepG2 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse skeletal muscle tissue lysate
Lane 7: Rat skeletal muscle tissue lysate
Lane 8: Rat brain tissue lysate
Lane 9: CHO whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-BHLH3 Antibody (N-term) (M04668)
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