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Product Info Summary
| SKU: | A10502 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BHLHA15 Antibody
SKU/Catalog Number
A10502
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BHLHA15 Antibody (Catalog # A10502). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BHLHA15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-BHLHA15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10502)
Host
Rabbit
Contents
BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15. The immunogen is located within amino acids 60 - 110 of BHLHA15.
Cross-reactivity
BHLHA15 antibody is human, mouse and rat reactive.
Reactive Species
A10502 is reactive to BHLHA15 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
20.8 kDa
Background of BHLHA15
BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10502 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BHLHA15 in rat small Intestine tissue lysate with BHLHA15 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide
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Immunohistochemistry of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 5 μg/mL.
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Immunofluorescence of BHLHA15 in human small intestine tissue with BHLHA15 antibody at 20 μg/mL.
Specific Publications For Anti-BHLHA15 Antibody (A10502)
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