Click image to see more details
Product Info Summary
| SKU: | A04668 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BHLHB3 BHLHE41 Antibody
SKU/Catalog Number
A04668
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BHLHB3 BHLHE41 Antibody (Catalog# A04668). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BHLHB3 BHLHE41 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04668)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BHLHB3. Immunogen sequence location: 1-50
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04668 is reactive to BHLHE41 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
50.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04668 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Click image to see more details
Western blot analysis of lysates from RAW264.7 cells, using BHLHB3 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BHLHB3 BHLHE41 Antibody (A04668)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-BHLHB3 BHLHE41 Antibody
Question
you antibody to test anti-BHLHB3 antibody A04668 on mouse spinal cord for research purposes, then I may be interested in using anti-BHLHB3 antibody A04668 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The products we sell, including anti-BHLHB3 antibody A04668, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-14
Question
Is a blocking peptide available for product anti-BHLHB3 antibody (A04668)?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
We do provide the blocking peptide for product anti-BHLHB3 antibody (A04668). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-27
Question
Does A04668 anti-BHLHB3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
As indicated on the product datasheet, A04668 anti-BHLHB3 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-20
Question
Does anti-BHLHB3 antibody A04668 work on zebrafish WB with spinal cord?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Our lab technicians have not tested anti-BHLHB3 antibody A04668 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-BHLHB3 antibody A04668 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish spinal cord in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-21
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spinal cord using anti-BHLHB3 antibody A04668. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-20
Question
We are currently using anti-BHLHB3 antibody A04668 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The anti-BHLHB3 antibody (A04668) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-12
Question
I see that the anti-BHLHB3 antibody A04668 works with WB, what is the protocol used to produce the result images on the product page?
R. Anderson
Verified customer
Asked: 2019-06-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-19
Question
Is this A04668 anti-BHLHB3 antibody reactive to the isotypes of BHLHE41?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
The immunogen of A04668 anti-BHLHB3 antibody is A peptide derived from human BHLHB3. Immunogen sequence location: 1-50. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-30
Question
Will anti-BHLHB3 antibody A04668 work for WB with spinal cord?
A. Lewis
Verified customer
Asked: 2018-04-05
Answer
According to the expression profile of spinal cord, BHLHE41 is highly expressed in spinal cord. So, it is likely that anti-BHLHB3 antibody A04668 will work for WB with spinal cord.
Boster Scientific Support
Answered: 2018-04-05
Question
I have attached the WB image, lot number and protocol we used for spinal cord using anti-BHLHB3 antibody A04668. Please let me know if you require anything else.
S. Patel
Verified customer
Asked: 2017-08-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-04
Question
I have a question about product A04668, anti-BHLHB3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Gonzalez
Verified customer
Asked: 2017-01-09
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04668 anti-BHLHB3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-01-09
Question
Is there a BSA free version of anti-BHLHB3 antibody A04668 available?
N. Krishna
Verified customer
Asked: 2015-08-14
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-BHLHB3 antibody A04668 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-08-14
Question
I was wanting to use your anti-BHLHB3 antibody for WB for mouse spinal cord on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse spinal cord identification?
D. Yang
Verified customer
Asked: 2015-04-28
Answer
You can see on the product datasheet, A04668 anti-BHLHB3 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse spinal cord in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-04-28
