Product Info Summary
SKU: | A06712-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-BHMT Antibody Picoband™
SKU/Catalog Number
A06712-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BHMT Antibody Picoband™ catalog # A06712-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BHMT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06712-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BHMT recombinant protein (Position: E80-Q406).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06712-1 is reactive to BHMT in Human, Monkey, Mouse, Rat
Applications
A06712-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
44.998kDa
Background of BHMT
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst (e)inemia, but such a defect has not yet been observed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Mouse, Rat, Monkey
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BHMT using anti-BHMT antibody (A06712-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: monkey liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BHMT antigen affinity purified polyclonal antibody (Catalog # A06712-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BHMT at approximately 45KD. The expected band size for BHMT is at 45KD.
Click image to see more details
Figure 2. Flow Cytometry analysis of HEPG2 cells using anti-BHMT antibody (A06712-1).
Overlay histogram showing HEPG2 cells stained with A06712-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BHMT Antibody (A06712-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of U937 cells using anti-BHMT antibody (A06712-1).
Overlay histogram showing U937 cells stained with A06712-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BHMT Antibody (A06712-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For BHMT (Source: Uniprot.org, NCBI)
Gene Name
BHMT
Full Name
Betaine--homocysteine S-methyltransferase 1
Weight
44.998kDa
Alternative Names
betaine homocysteine methyltransferase; betaine-homocysteine methyltransferase; betaine--homocysteine S-methyltransferase 1; betaine--homocysteine S-methyltransferase; BHMT1; EC 2.1.1; EC 2.1.1.5 BHMT BHMT1, HEL-S-61p betaine--homocysteine S-methyltransferase betaine--homocysteine S-methyltransferase 1|epididymis secretory sperm binding protein Li 61p
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BHMT, check out the BHMT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BHMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BHMT Antibody Picoband™ (A06712-1)
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No publications found for A06712-1
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