Click image to see more details
-
-
-
-
-
+7
Product Info Summary
| SKU: | A01552 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Bim BCL2L11 Antibody
SKU/Catalog Number
A01552
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bim BCL2L11 Antibody (Catalog # A01552). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Bim antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bim BCL2L11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01552)
Host
Rabbit
Contents
Bim Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-BIM antibody was raised against a peptide corresponding to amino acids near the center of human BIM. The immunogen is located within amino acids 80-130 of BIM.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of TLR8 are known to exist. TLR8 antibody is predicted to not cross-react with other TLR protein family members
Reactive Species
A01552 is reactive to BCL2L11 in Human, Mouse
Applications
A01552 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
22.171kDa
Background of BCL2L11
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 5 μg/mL; ICC: 10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: BIM A01552, (5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: BIM, (0.5 μg/mL), BIM A01552, (5 μg/mL), beta-actin (1 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Click image to see more details
Western Blot Validation in Human Tissue
Loading: 15 μg of lysates per lane. Antibodies: BIM A01552, (5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: Human urinary bladderLane 2: Human pancreas
Click image to see more details
Immunofluorescence Validation of BIM in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling BIM with A01552 at 20 μg/mL
Click image to see more details
Immunocytochemistry Validation of BIM K562 Cells
Immunohistochemical analysis of K562 cells using anti-BIM antibody (A01552) at 10 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Click image to see more details
Induced Expression Validation of BIM in Mouse Hippocampus (Tsuchiya et al.
Click image to see more details
KD Validation of BIM in 293 Cells (Han et al.
Click image to see more details
Regulated Expression Validation of BIM in U266 Cells (Chen et al.
Click image to see more details
WB KD Validation of BIM in 293 Cells (Han et al.
Click image to see more details
KD Validation of BIM in Human Cell Lines (Chen et al.
Click image to see more details
Localization Validation of BIM in Mouse Macrophages (Ulett et al.
Protein Target Info & Infographic
Gene/Protein Information For BCL2L11 (Source: Uniprot.org, NCBI)
Gene Name
BCL2L11
Full Name
Bcl-2-like protein 11
Weight
22.171kDa
Superfamily
Bcl-2 family
Alternative Names
BAM; bcl-2 interacting mediator of cell death; bcl-2 interacting protein Bim; Bcl2-interacting mediator of cell death; bcl2-L-11; BCL2-like 11 (apoptosis facilitator); bcl-2-like protein 11; bcl-2-related ovarian death agonist; BIM-alpha6; BIM-beta6; BIM-beta7; BIMBOD; BimEL; BimL BCL2L11 BAM, BIM, BOD BCL2 like 11 bcl-2-like protein 11|BCL2-like 11 (apoptosis facilitator)|bcl-2 interacting mediator of cell death|bcl-2 interacting protein Bim|bcl-2-related ovarian death agonist
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL2L11, check out the BCL2L11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL2L11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bim BCL2L11 Antibody (A01552)
Hello CJ!
No publications found for A01552
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Bim BCL2L11 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Bim BCL2L11 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
