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Product Info Summary
| SKU: | A01552-5 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Bim/Bcl2l11 Antibody Picoband®
SKU/Catalog Number
A01552-5
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bim/Bcl2l11 Antibody Picoband® catalog # A01182-4. Tested in WB, FCM, ELISA applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Bim/Bcl2l11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01552-5)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived mouse Bim/Bcl2l11 recombinant protein (Position: Q18-R186). Mouse Bcl2l11 shares 86.7% and 98.8% amino acid (aa) sequence identity with human and rat Bcl2l11, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01552-5 is reactive to Bcl2l11 in Mouse
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.1 kDa
Background of Bcl2l11
Bcl-2-like protein 11, commonly called BIM (Bcl-2?Interacting?Mediator of cell death), is a?protein?that in humans is encoded by the?BCL2L11?gene. BCL2 is a prototypic mammalian regulator of cell death. Certain members of the BCL2 family inhibit apoptosis, while other members facilitate this physiologic process of cell death. By screening a cDNA expression library using recombinant BCL2 as bait, O'Connor et al. isolated a small novel protein, which they referred to as BIM, with a 9-amino acid BH3 motif shared by most BCL2 homologs. BIM provokes apoptosis, and the BH3 region is required for BCL2 binding and for most of its cytotoxicity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01552-5 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: mouse spleen tissue, mouse thymus tissue
FCM: C2C12 cell
Validation Images & Assay Conditions
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Western blot analysis of Bim/Bcl2l11 using anti-Bim/Bcl2l11 antibody (A01552-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bim/Bcl2l11 antigen affinity purified polyclonal antibody (Catalog # A01552-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bim/Bcl2l11 at approximately 22 kDa. The expected band size for Bim/Bcl2l11 is at 22 kDa.
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Flow Cytometry analysis of C2C12 cells using anti-Bim/Bcl2l11 antibody (A01552-5).
Overlay histogram showing C2C12 cells stained with A01552-5 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bim/Bcl2l11 Antibody (A01552-5, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-Bim/Bcl2l11 Antibody Picoband® (A01552-5)
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