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Product Info Summary
| SKU: | A02459 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Bit1 PTRH2 Antibody
SKU/Catalog Number
A02459
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bit1 PTRH2 Antibody (Catalog # A02459). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Bit1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bit1 PTRH2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02459)
Host
Rabbit
Contents
Bit1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bit1 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Bit1. The immunogen is located within amino acids 20 - 70 of Bit1.
Reactive Species
A02459 is reactive to PTRH2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
19.2 kDa
Background of PTRH2
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02459 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Recommend Dilution
Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bit1 in A-20 cell lysate with Bit1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
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Immunocytochemistry of Bit1 in 3T3 cells with Bit1 antibody at 2 μg/mL.
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Immunofluorescence of Bit1 in 3T3 cells with Bit1 antibody at 20 μg/mL.
Specific Publications For Anti-Bit1 PTRH2 Antibody (A02459)
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