Anti-BMI-1 Antibody

Product Name

Anti-BMI-1 Antibody

View all BMI1 Antibodies

SKU/Catalog Number

A00313

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BMI-1 Antibody (Catalog # A00313). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

BMI-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BMI-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00313)

Host

Rabbit

Contents

BMI-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1. The immunogen is located within amino acids 140 - 190 of BMI-1.

Reactive Species

A00313 is reactive to BMI1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

36.9 kDa

Background of BMI1

The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence. Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells, suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00313 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BMI-1 antibody can be used for detection of BMI-1 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BMI1

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