Product Info Summary
SKU: | M00313 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bmi1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00313
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bmi1 Rabbit Monoclonal Antibody catalog # M00313. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bmi1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00313)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IHE-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bmi1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00313 is reactive to BMI1 in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
40 kDa
Calculated molecular weight
36949 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00313 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human K562 whole cell, human U2OS whole cell, human A431 whole cell, human A549 whole cell, rat PC-12 whole cell, rat C6 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Bmi1 using anti-Bmi1 antibody (M00313).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates,
Lane 6: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bmi1 antigen affinity purified monoclonal antibody (Catalog # M00313) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bmi1 at approximately 40 kDa. The expected band size for Bmi1 is at 37 kDa.
Protein Target Info & Infographic
Gene/Protein Information For BMI1 (Source: Uniprot.org, NCBI)
Gene Name
BMI1
Full Name
Polycomb complex protein BMI-1
Weight
36949 MW
Alternative Names
Polycomb complex protein BMI-1;Polycomb group RING finger protein 4;RING finger protein 51;BMI1;PCGF4, RNF51; BMI1 FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1 BMI1 proto-oncogene, polycomb ring finger polycomb complex protein BMI-1|B lymphoma Mo-MLV insertion region 1 homolog|BMI1 polycomb ring finger oncogene|BMI1 polycomb ring finger proto-oncogene|murine leukemia viral (bmi-1) oncogene homolog|polycomb group RING finger protein 4|polycomb group protein Bmi1|ring finger protein 51
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMI1, check out the BMI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bmi1 Rabbit Monoclonal Antibody (M00313)
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