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Product Info Summary
| SKU: | A02537 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-BMX Antibody
SKU/Catalog Number
A02537
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-BMX Antibody (Catalog # A02537). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02537)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human BMX.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02537 is reactive to BMX in Human, Mouse
Reconstitution
Calculated molecular weight
78011 MW
Background of BMX
Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.
Tamagnone L., Oncogene 9:3683-3688(1994).
Qiu Y., Proc. Natl. Acad. Sci. U.S.A. 95:3644-3649(1998).
Fuortes M., Thesis (1994), Cornell University, United States.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02537 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using BMX antibody A02537.
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Western blot analysis of extracts from COS-7 cells, using BMX antibody A02537.
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Immunofluorescence analysis of A549 cells, using BMX antibody A02537.
Protein Target Info & Infographic
Gene/Protein Information For BMX (Source: Uniprot.org, NCBI)
Gene Name
BMX
Full Name
Cytoplasmic tyrosine-protein kinase BMX
Weight
78011 MW
Superfamily
protein kinase superfamily
Alternative Names
Cytoplasmic tyrosine-protein kinase BMX;2.7.10.2;Bone marrow tyrosine kinase gene in chromosome X protein;Epithelial and endothelial tyrosine kinase;ETK;NTK38;BMX; BMX ETK, PSCTK2, PSCTK3 BMX non-receptor tyrosine kinase cytoplasmic tyrosine-protein kinase BMX|BTK-like on X chromosome|Etk/Bmx cytosolic tyrosine kinase|NTK38 tyrosine kinase|bone marrow tyrosine kinase gene in chromosome X protein|epithelial and endothelial tyrosine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMX, check out the BMX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMX Antibody (A02537)
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No publications found for A02537
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-BMX Antibody
Question
We are currently using anti-BMX antibody A02537 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
The anti-BMX antibody (A02537) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-21
Question
See attached the WB image, lot number and protocol we used for bone marrow using anti-BMX antibody A02537. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
Will A02537 anti-BMX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
You can see on the product datasheet, A02537 anti-BMX antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-01
