Click image to see more details
Product Info Summary
| SKU: | A30599 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-BORG1 CDC42EP2 Antibody
SKU/Catalog Number
A30599
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BORG1 CDC42EP2 Antibody (Catalog# A30599). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BORG1 CDC42EP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30599)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BORG1. Immunogen sequence location: 10-59
Reactive Species
A30599 is reactive to CDC42EP2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
22.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30599 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Click image to see more details
Western Blot analysis of K562 cells using Cdc42EP2 Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from K562 cells, using BORG1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BORG1 CDC42EP2 Antibody (A30599)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-BORG1 CDC42EP2 Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-BORG1 CDC42EP2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-BORG1 CDC42EP2 Antibody
Question
I have a question about product A30599, anti-BORG1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30599 anti-BORG1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-01
Question
Is this A30599 anti-BORG1 antibody reactive to the isotypes of CDC42EP2?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The immunogen of A30599 anti-BORG1 antibody is A peptide derived from human BORG1. Immunogen sequence location: 10-59. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-BORG1 antibody A30599 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
The anti-BORG1 antibody (A30599) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-05
Question
Would anti-BORG1 antibody A30599 work for WB with lung pancreas?
E. Zhang
Verified customer
Asked: 2016-05-12
Answer
According to the expression profile of lung pancreas, CDC42EP2 is highly expressed in lung pancreas. So, it is likely that anti-BORG1 antibody A30599 will work for WB with lung pancreas.
Boster Scientific Support
Answered: 2016-05-12
Question
Does anti-BORG1 antibody A30599 work on bovine WB with brain?
G. Moore
Verified customer
Asked: 2016-05-06
Answer
Our lab technicians have not validated anti-BORG1 antibody A30599 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-BORG1 antibody A30599 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-06
Question
Will A30599 anti-BORG1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Johnson
Verified customer
Asked: 2016-03-25
Answer
As indicated on the product datasheet, A30599 anti-BORG1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-25
