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Product Info Summary
| SKU: | A30600 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BORG4 CDC42EP4 Antibody
SKU/Catalog Number
A30600
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BORG4 CDC42EP4 Antibody (Catalog# A30600). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BORG4 CDC42EP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30600)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BORG4. Immunogen sequence location: 201-250
Reactive Species
A30600 is reactive to CDC42EP4 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
38.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30600 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of lysates from Jurkat cells, using BORG4 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BORG4 CDC42EP4 Antibody (A30600)
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Customer Q&As
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3 Customer Q&As for Anti-BORG4 CDC42EP4 Antibody
Question
I was wanting to use your anti-BORG4 antibody for WB for human colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
As indicated on the product datasheet, A30600 anti-BORG4 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-11
Question
Does anti-BORG4 antibody A30600 work on goat WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
Our lab technicians have not tested anti-BORG4 antibody A30600 on goat. You can run a BLAST between goat and the immunogen sequence of anti-BORG4 antibody A30600 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-21
Question
We are currently using anti-BORG4 antibody A30600 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The anti-BORG4 antibody (A30600) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-28
