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Product Info Summary
| SKU: | A14584 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-BRAL1 HAPLN2 Antibody
SKU/Catalog Number
A14584
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-BRAL1 HAPLN2 Antibody (Catalog # A14584). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
BRAL1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-BRAL1 HAPLN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14584)
Host
Rabbit
Contents
BRAL1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BRAL1 antibody was raised against a 12 amino acid synthetic peptide from near the center of human BRAL1. The immunogen is located within amino acids 130 - 180 of BRAL1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A14584 is reactive to HAPLN2 in Human, Mouse, Rat
Applications
A14584 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
37.775kDa
Background of HAPLN2
BRAL1 is a member a superfamily consisting of several highly homologous hyaluronan and proteoglycan binding link proteins. BRAL1 is predominantly expressed in brain tissue and spinal cord. Like other members in the link-module superfamily, BRAL1 contains an immunoglobulin-like fold and two proteoglycan tandem repeats (PTRs). Its mRNA expression pattern is similar to other lectican proteoglycans, suggesting that BRAL1 may act to stabilize the binding between the extracellular matrix molecule hyaluronan and brevican. Immunostaining of mouse brain showed BRAL1 expression at P20 in the white matter of the developing cerebellum and in myelinated fiber tracts in the adult brain, suggesting that expression starts when axonal myelination occurs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BRAL1 antibody can be used for the detection of BRAL1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of BRAL1 in human brain tissue lysate with BRAL1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemical staining of human brain tissue using BRAL1 antibody at 2.5 μg/mL.
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Immunofluorescence of BRAL1 in Human Brain cells with BRAL1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For HAPLN2 (Source: Uniprot.org, NCBI)
Gene Name
HAPLN2
Full Name
Hyaluronan and proteoglycan link protein 2
Weight
37.775kDa
Superfamily
HAPLN family
Alternative Names
Brain link protein 1; brain link protein-1; BRAL1; hyaluronan and proteoglycan link protein 2 HAPLN2 BRAL1 hyaluronan and proteoglycan link protein 2 hyaluronan and proteoglycan link protein 2|brain link protein-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HAPLN2, check out the HAPLN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HAPLN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRAL1 HAPLN2 Antibody (A14584)
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No publications found for A14584
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Customer Reviews
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