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Product Info Summary
| SKU: | M02247 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Olig2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02247
BM3996 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Olig2 Rabbit Monoclonal Antibody catalog # M02247. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Olig2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02247)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADC-15
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Olig2
Reactive Species
M02247 is reactive to OLIG2 in Human, Mouse, Rat
Observed Molecular Weight
36 kDa
Calculated molecular weight
32.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02247 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human HEL whole cell, rat brain tissue, mouse brain tissue
IHC: human mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of OLIG2 using anti-OLIG2 antibody (M02247).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLIG2 antigen affinity purified monoclonal antibody (M02247) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OLIG2 at approximately 36 kDa. The expected band size for OLIG2 is at 32 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human mouse brain, using Olig2 Antibody.
Specific Publications For Anti-Olig2 Rabbit Monoclonal Antibody (M02247)
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4 Customer Q&As for Anti-Olig2 Rabbit Monoclonal Antibody
Question
Does M02247 anti-Olig2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can see on the product datasheet, M02247 anti-Olig2 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-10
Question
Do you have a BSA free version of anti-Olig2 Rabbit Monoclonal antibody M02247 available?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Olig2 Rabbit Monoclonal antibody M02247 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-06
Question
I see that the anti-Olig2 Rabbit Monoclonal antibody M02247 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-03-21
Question
Will anti-Olig2 Rabbit Monoclonal antibody M02247 work on canine WB with globus pallidus?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
Our lab technicians have not tested anti-Olig2 Rabbit Monoclonal antibody M02247 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Olig2 Rabbit Monoclonal antibody M02247 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine globus pallidus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-04
