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Product Info Summary
| SKU: | A08515 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BRWD3 Antibody
SKU/Catalog Number
A08515
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BRWD3 Antibody (Catalog# A08515). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRWD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08515)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BRWD3. Immunogen sequence location: 1751-1800
Reactive Species
A08515 is reactive to BRWD3 in Human, Mouse
Observed Molecular Weight
72 kDa
Calculated molecular weight
203.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08515 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:40000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using BRWD3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO cells, using BRWD3 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BRWD3 Antibody (A08515)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-BRWD3 Antibody
Question
Is this A08515 anti-BRWD3 antibody reactive to the isotypes of BRWD3?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
The immunogen of A08515 anti-BRWD3 antibody is A peptide derived from human BRWD3. Immunogen sequence location: 1751-1800. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-11
Question
We are currently using anti-BRWD3 antibody A08515 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The anti-BRWD3 antibody (A08515) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-24
Question
I was wanting to use to test anti-BRWD3 antibody A08515 on human cervix carcinoma for research purposes, then I may be interested in using anti-BRWD3 antibody A08515 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Krishna
Verified customer
Asked: 2017-03-14
Answer
The products we sell, including anti-BRWD3 antibody A08515, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-14
Question
I have a question about product A08515, anti-BRWD3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Evans
Verified customer
Asked: 2013-01-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08515 anti-BRWD3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-01-29
