Anti-Bub1 AAMP Antibody Picoband™

AAMP antibody

Boster Bio Anti-Bub1 AAMP Antibody Picoband™ catalog # PB9135. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9135
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Bub1 AAMP Antibody Picoband™

View all AAMP Antibodies

SKU/Catalog Number

PB9135

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bub1 AAMP Antibody Picoband™ catalog # PB9135. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bub1 AAMP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9135)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9135 is reactive to AAMP in Human, Mouse, Rat

Applications

PB9135 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

130 kDa

Calculated molecular weight

46.751kDa

Background of AAMP

BUB1, also known as mitotic checkpoint serine/threonine kinase, is an enzyme that in humans is encoded by the BUB1 gene. It is mapped to 2q13. BUB1 is first identified in genetic screens of Saccharomyces cerevisiae. The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression. The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans. Loss-of-function mutations or absence of BUB1 has been reported to result in aneuploidy, chromosomal instability (CIN) and premature senescence. The protein kinase BUB1 possesses versatile and distinct functions during the cell cycle, mainly in the SAC and chromosome alignment during metaphase.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For AAMP (Source: Uniprot.org, NCBI)

Gene Name

AAMP

Full Name

Angio-associated migratory cell protein

Weight

46.751kDa

Alternative Names

angio-associated migratory cell protein; angio-associated, migratory cell protein AAMP angio associated migratory cell protein angio-associated migratory cell protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on AAMP, check out the AAMP Infographic

AAMP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for AAMP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-Bub1 AAMP Antibody Picoband™

Question

I see that the anti-Bub1 antibody PB9135 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-14

Question

Is a blocking peptide available for product anti-Bub1 antibody (PB9135)?

Verified Customer

Verified customer

Asked: 2019-05-08

Answer

We do provide the blocking peptide for product anti-Bub1 antibody (PB9135). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-05-08

Question

We are currently using anti-Bub1 antibody PB9135 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-01-17

Answer

The anti-Bub1 antibody (PB9135) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-01-17

Question

My question regarding product PB9135, anti-Bub1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9135 anti-Bub1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-06

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Bub1 antibody PB9135. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-11-29

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-29

Size

Conjugation

Total: $370

SKU:PB9135

In stock, 3+ left.

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PB9135
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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