|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-Bub1 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Mitotic checkpoint serine/threonine-protein kinase BUB1(BUB1) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-Bub1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9135)|
|Replacement Item||This antibody may replace the following items: sc-18286|sc-31100|sc-28257|sc-31101|sc-365685|sc-47743|sc-22494 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Bub1 recombinant protein (Position: V731-K1085). Human Bub1 shares 81% amino acid (aa) sequence identity with mouse Bub1.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mitotic checkpoint serine/threonine-protein kinase BUB1|
|Molecular Weight||46751 MW|
|Protein Function||Plays a role in angiogenesis and cell migration. In smooth muscle cell migration, may act through the RhoA pathway. .|
|Tissue Specificity||Expressed in metastatic melanoma, liver, skin, kidney, heart, lung, lymph node, skeletal muscle and brain, and also in A2058 melanoma cells and activated T-cells (at protein level). Expressed in blood vessels. Strongly expressed in endothelial cells, cytotrophoblasts, and poorly differentiated. colon adenocarcinoma cells found in lymphatics. .|
|Sequence Similarities||Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.|
|Subcellular Localization||Cell membrane. Cytoplasm.|
|Alternative Names||Angio-associated migratory cell protein;AAMP;|
|Research Areas|||cell biology|cell cycle|cell division|spindle| cancer||
Background for Mitotic checkpoint serine/threonine-protein kinase BUB1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Bub1 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Bub1 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti BUB1 (PB9135) at 0.5ug/ml
WB: Recombinant Human BUB1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti BUB1 (PB9135) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 122KD
Observed bind size: 122KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,