Anti-C Reactive Protein/Crp Antibody Picoband™

Boster Bio Anti-C Reactive Protein/Crp Antibody Picoband™ catalog # A00249-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Product Info Summary

SKU: A00249-2
Size: 100μg/vial
Reactive Species: Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-C Reactive Protein/Crp Antibody Picoband™

See all C-Reactive Protein/CRP products

SKU/Catalog Number

A00249-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-C Reactive Protein/Crp Antibody Picoband™ catalog # A00249-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C Reactive Protein/Crp Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00249-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived rat C Reactive Protein recombinant protein (Position: H20-S230).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00249-2 is reactive to Crp in Rat

Applications

A00249-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For Crp (Source: Uniprot.Org, NCBI)

Uniprot ID

P48199

Gene ID

25419

Gene Name

Crp

Full Name

C-reactive protein

Weight

25.468kDa

Superfamily

pentraxin family

Alternative Names

C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895 Crp|Aa1249, Ab1-341, Ab2-196, Ac1-114, Ac1262, Ac2-069, Ba2-693|C-reactive protein|C-reactive protein|C-reactive protein, member of the pentraxin family|C-reactive protein, pentraxin-related|C-reactive protein, petaxin related

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Crp, check out the Crp Infographic

Crp infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Crp: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A00249-2

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16 Customer Q&As for Anti-C Reactive Protein/Crp Antibody Picoband™

Question

My question regarding product A00249-2, anti-C Reactive Protein/Crp antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00249-2 anti-C Reactive Protein/Crp antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-18

Question

My colleagues were happy with the WB result of your anti-C Reactive Protein/Crp antibody. However we have observed positive staining in urinary bladder secreted. using this antibody. Is that expected? Could you tell me where is CRP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-19

Answer

Based on literature, urinary bladder does express CRP. Generally CRP expresses in secreted. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-12-19

Question

Will A00249-2 anti-C Reactive Protein/Crp antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

As indicated on the product datasheet, A00249-2 anti-C Reactive Protein/Crp antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-03

Question

We ordered your anti-C Reactive Protein/Crp antibody for WB on liver a few years ago. I am using rat, and We want to use the antibody for ELISA next. I am looking for examining liver as well as urinary bladder in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

I took a look at the website and datasheets of our anti-C Reactive Protein/Crp antibody and I see that A00249-2 has been validated on rat in both WB and ELISA. Thus A00249-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-20

Question

Is there a BSA free version of anti-C Reactive Protein/Crp antibody A00249-2 available?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-C Reactive Protein/Crp antibody A00249-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-C Reactive Protein/Crp antibody A00249-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-11

Question

Would anti-C Reactive Protein/Crp antibody A00249-2 work for ELISA with liver?

Verified Customer

Verified customer

Asked: 2019-10-31

Answer

According to the expression profile of liver, CRP is highly expressed in liver. So, it is likely that anti-C Reactive Protein/Crp antibody A00249-2 will work for ELISA with liver.

Boster Scientific Support

Answered: 2019-10-31

Question

you antibody using your anti-C Reactive Protein/Crp antibody for wound healing studies. Has this antibody been tested with western blotting on rat cerebellum tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-08-21

Answer

Thanks for your inquiry. This A00249-2 anti-C Reactive Protein/Crp antibody is validated on rat cerebellum tissue, heart tissue, lung tissue, spleen tissue. It is guaranteed to work for ELISA, IHC, WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-08-21

Question

Is this A00249-2 anti-C Reactive Protein/Crp antibody reactive to the isotypes of CRP?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

The immunogen of A00249-2 anti-C Reactive Protein/Crp antibody is E. coli-derived rat C Reactive Protein recombinant protein (Position: H20-S230). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-24

Question

I see that the anti-C Reactive Protein/Crp antibody A00249-2 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-21

Question

Is a blocking peptide available for product anti-C Reactive Protein/Crp antibody (A00249-2)?

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

We do provide the blocking peptide for product anti-C Reactive Protein/Crp antibody (A00249-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-14

Question

We are currently using anti-C Reactive Protein/Crp antibody A00249-2 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-03-22

Answer

The anti-C Reactive Protein/Crp antibody (A00249-2) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-03-22

Question

I was wanting to use your anti-C Reactive Protein/Crp antibody for ELISA for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?

Verified Customer

Verified customer

Asked: 2018-07-25

Answer

You can see on the product datasheet, A00249-2 anti-C Reactive Protein/Crp antibody has been validated for ELISA, IHC, WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-25

Question

I would like to test anti-C Reactive Protein/Crp antibody A00249-2 on rat liver for research purposes, then I may be interested in using anti-C Reactive Protein/Crp antibody A00249-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-10-12

Answer

The products we sell, including anti-C Reactive Protein/Crp antibody A00249-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-10-12

Question

We have observed staining in rat right lobe of liver. What should we do? Is anti-C Reactive Protein/Crp antibody supposed to stain right lobe of liver positively?

A. Huang

Verified customer

Asked: 2014-12-23

Answer

Based on literature right lobe of liver does express CRP. Based on Uniprot.org, CRP is expressed in right lobe of liver, liver, urinary bladder, among other tissues. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2014-12-23

Question

I have attached the WB image, lot number and protocol we used for liver using anti-C Reactive Protein/Crp antibody A00249-2. Please let me know if you require anything else.

D. Miller

Verified customer

Asked: 2013-08-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-08-16

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00249-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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