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Product Info Summary
| SKU: | M00249 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody
SKU/Catalog Number
M00249
BM4227 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody catalog # M00249. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00249)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CFI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human C Reactive Protein
Reactive Species
M00249 is reactive to CRP in Human
Observed Molecular Weight
25 kDa
Calculated molecular weight
25.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00249 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human hepatocellular carcinoma tumor tissue, human hepatocellular carcinoma paracancerous tissue
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of CRP using anti-CRP antibody (M00249).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRP antigen affinity purified monoclonal antibody (M00249) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CRP at approximately 25 kDa. The expected band size for CRP is at 25 kDa.
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IHC analysis of CRP using anti-CRP antibody (M00249).
CRP was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CRP Antibody (M00249) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody (M00249)
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Customer Q&As
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4 Customer Q&As for Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody
Question
We are currently using anti-C Reactive Protein Rabbit Monoclonal antibody M00249 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-C Reactive Protein Rabbit Monoclonal antibody (M00249) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
We have tried in the past anti-C Reactive Protein Rabbit Monoclonal antibody for IP on liver in a previous project. I am using human, and We intend to use the antibody for IHC next. We need examining liver as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
L. Mitchell
Verified customer
Asked: 2019-07-15
Answer
I looked at the website and datasheets of our anti-C Reactive Protein Rabbit Monoclonal antibody and it appears that M00249 has been validated on human in both IP and IHC. Thus M00249 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-15
Question
Our lab were happy with the WB result of your anti-C Reactive Protein Rabbit Monoclonal antibody. However we have seen positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is CRP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
Based on literature, liver does express CRP. Generally CRP expresses in secreted. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-05-18
Question
We have seen staining in human right lobe of liver. Are there any suggestions? Is anti-C Reactive Protein Rabbit Monoclonal antibody supposed to stain right lobe of liver positively?
K. Krishna
Verified customer
Asked: 2013-02-08
Answer
Based on literature right lobe of liver does express CRP. Based on Uniprot.org, CRP is expressed in right lobe of liver, liver, urinary bladder, among other tissues. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-02-08
