Overview
| Product Name |
Anti-C19orf80/ANGPTL8 Antibody Picoband™
See more Angiopoietin-like protein 8/Betatrophin products |
| Catalog# |
A02471 |
| Pack Size |
100ug/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-C19orf80/ANGPTL8 Antibody Picoband™ catalog # A02471. Tested in WB applications. This antibody reacts with Human. Supplied as 100ug/vial in Lyophilized form antibody. |
| Cite This Product |
Anti-C19orf80/ANGPTL8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02471)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E.coli-derived human C19orf80 recombinant protein (Position: A22-A198). Human C19orf80 shares 74% amino acid (aa) sequence identity with mouse C19orf80. |
| Cross Reactivity |
No cross reactivity with other proteins. |
Gene/Protein Basic Information For ANGPTL8 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q6UXH0 |
|---|
| NCBI Gene Id | 55908 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | ANGPTL8 |
|---|
| Protein Name | Angiopoietin-like protein 8 |
|---|
| Superfamily | ANGPTL8 family |
|---|
| Alternative Names | Angiopoietin-like protein 8/Betatrophin|Angiopoietin like Protein 8; Angiopoietin-like Protein 8; ANGPTL8; Betatrophin; C19orf80; chromosome 19 open reading frame 80; Gm6484; hepatocellular carcinoma-associated gene TD26; hepatocellular carcinoma-associated protein TD26; Lipasin; PRO1185; PVPA599; RIFL; TD26; UNQ599 |
|---|
| Molecular Weight | 22105 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ANGPTL8, check out the ANGPTL8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ANGPTL8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ANGPTL8 infographic
Our Boster Quality Guarantee for Anti-C19orf80/ANGPTL8 Antibody Picoband™ covers its use in the following applications.
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Figure 1. Western blot analysis of C19orf80 using anti-C19orf80 antibody (A02471).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf80 antigen affinity purified polyclonal antibody (Catalog # A02471) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf80 at approximately 22KD. The expected band size for C19orf80 is at 22KD.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
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Questions and answers from customers related to A02471 Anti-C19orf80/ANGPTL8 Antibody Picoband™
7 Related Questions
Question
Is a blocking peptide available for product anti-C19orf80/ANGPTL8 antibody (A02471)?
Verified Customer
Asked: 2020-03-23
Answer
We do provide the blocking peptide for product anti-C19orf80/ANGPTL8 antibody (A02471). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-23
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-C19orf80/ANGPTL8 antibody A02471. Let me know if you need anything else.
Verified Customer
Asked: 2020-02-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-14
Question
We are currently using anti-C19orf80/ANGPTL8 antibody A02471 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Asked: 2019-12-02
Answer
The anti-C19orf80/ANGPTL8 antibody (A02471) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-02
Question
Do you have a BSA free version of anti-C19orf80/ANGPTL8 antibody A02471 available?
Verified Customer
Asked: 2019-10-15
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-C19orf80/ANGPTL8 antibody A02471 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-15
Question
I see that the anti-C19orf80/ANGPTL8 antibody A02471 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Asked: 2019-05-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-24
Question
Would anti-C19orf80/ANGPTL8 antibody A02471 work on primate WB with liver?
Verified Customer
Asked: 2019-05-15
Answer
Our lab technicians have not validated anti-C19orf80/ANGPTL8 antibody A02471 on primate. You can run a BLAST between primate and the immunogen sequence of anti-C19orf80/ANGPTL8 antibody A02471 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-15
Question
I was wanting to use your anti-C19orf80/ANGPTL8 antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Asked: 2018-09-19
Answer
You can see on the product datasheet, A02471 anti-C19orf80/ANGPTL8 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-19
