Anti-CA VB CA5B Antibody

Carbonic Anhydrase VB/CA5B antibody

Boster Bio Anti-CA VB CA5B Antibody catalog # A11974-1. Tested in ELISA, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A11974-1
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

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Product Name

Anti-CA VB CA5B Antibody

View all Carbonic Anhydrase VB/CA5B Antibodies

SKU/Catalog Number

A11974-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-CA VB CA5B Antibody catalog # A11974-1. Tested in ELISA, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CA VB CA5B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11974-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

3G2

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human CA5B at amino acid range 241-290

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11974-1 is reactive to CA5B in Human, Mouse, Rat

Applications

A11974-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

36.434kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000

Validation Images & Assay Conditions

Gene/Protein Information For CA5B (Source: Uniprot.org, NCBI)

Gene Name

CA5B

Full Name

Carbonic anhydrase 5B, mitochondrial

Weight

36.434kDa

Superfamily

alpha-carbonic anhydrase family

Alternative Names

CA5B; Carbonate dehydratase VB; carbonic anhydrase 5B, mitochondrial; Carbonic Anhydrase VB; carbonic anhydrase VB, mitochondrial; carbonic dehydratase; CAVB; CA-VB; EC 4.2.1.1; MGC39962 CA5B CA-VB, CAVB carbonic anhydrase 5B carbonic anhydrase 5B, mitochondrial|carbonate dehydratase VB|carbonic anhydrase VB, mitochondrial|carbonic dehydratase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CA5B, check out the CA5B Infographic

CA5B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CA5B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-CA VB CA5B Antibody

Question

Is a blocking peptide available for product anti-CA VB antibody (A11974-1)?

Verified Customer

Verified customer

Asked: 2020-04-21

Answer

We do provide the blocking peptide for product anti-CA VB antibody (A11974-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-21

Question

Will A11974-1 anti-CA VB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-28

Answer

It shows on the product datasheet, A11974-1 anti-CA VB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-28

Question

Can you help my question with product A11974-1, anti-CA VB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11974-1 anti-CA VB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-07

Question

My lab would like to test anti-CA VB antibody A11974-1 on human tendon of biceps brachii for research purposes, then I may be interested in using anti-CA VB antibody A11974-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-03-18

Answer

The products we sell, including anti-CA VB antibody A11974-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-18

Question

I was wanting to use your anti-CA VB antibody for WB for human tendon of biceps brachii on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tendon of biceps brachii identification?

Verified Customer

Verified customer

Asked: 2018-08-31

Answer

You can see on the product datasheet, A11974-1 anti-CA VB antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human tendon of biceps brachii in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-31

Question

Is this A11974-1 anti-CA VB antibody reactive to the isotypes of CA5B?

S. Anderson

Verified customer

Asked: 2016-03-10

Answer

The immunogen of A11974-1 anti-CA VB antibody is Synthesized peptide derived from the C-terminal region of human CA VB. at AA rangle: 210-290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-03-10

Order DetailsPrice
A11974-1

100uL

$399

Free Secondary Antibody

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A11974-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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