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Product Info Summary
| SKU: | A11974-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CA VB CA5B Antibody
SKU/Catalog Number
A11974-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CA VB CA5B Antibody catalog # A11974-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CA VB CA5B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11974-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CA5B. AA range:241-290
Reactive Species
A11974-1 is reactive to CA5B in Human, Mouse, Rat
Calculated molecular weight
36.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11974-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of lysates from NIH/3T3 cells, using CA5B Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HT-29 cells using CA5B antibody.
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of MCF7 cells, using CA5B Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-CA VB CA5B Antibody (A11974-1)
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Customer Q&As
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6 Customer Q&As for Anti-CA VB CA5B Antibody
Question
Is a blocking peptide available for product anti-CA VB antibody (A11974-1)?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
We do provide the blocking peptide for product anti-CA VB antibody (A11974-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-04-21
Question
Will A11974-1 anti-CA VB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
It shows on the product datasheet, A11974-1 anti-CA VB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-28
Question
Can you help my question with product A11974-1, anti-CA VB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11974-1 anti-CA VB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-07
Question
My lab would like to test anti-CA VB antibody A11974-1 on human tendon of biceps brachii for research purposes, then I may be interested in using anti-CA VB antibody A11974-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
The products we sell, including anti-CA VB antibody A11974-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-18
Question
I was wanting to use your anti-CA VB antibody for WB for human tendon of biceps brachii on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tendon of biceps brachii identification?
Verified Customer
Verified customer
Asked: 2018-08-31
Answer
You can see on the product datasheet, A11974-1 anti-CA VB antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human tendon of biceps brachii in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-31
Question
Is this A11974-1 anti-CA VB antibody reactive to the isotypes of CA5B?
S. Anderson
Verified customer
Asked: 2016-03-10
Answer
The immunogen of A11974-1 anti-CA VB antibody is Synthesized peptide derived from the C-terminal region of human CA VB. at AA rangle: 210-290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-03-10
