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Product Info Summary
| SKU: | A11254 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CaBP8 CALN1 Antibody
SKU/Catalog Number
A11254
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CaBP8 CALN1 Antibody (Catalog # A11254). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CaBP8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CaBP8 CALN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11254)
Host
Rabbit
Contents
CaBP8 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CaBP8 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CaBP8. The immunogen is located within the first 50 amino acids of CaBP8.
Reactive Species
A11254 is reactive to CALN1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
24.8 kDa
Background of CALN1
Calcium binding proteins (CaBP) play a crucial role in the calcium-mediated cellular signal transduction pathway in the central nervous system. The CaBP family shares much similarity with CaM I (calmodulin), and it has been shown that CaBP proteins can substitute functionally for, and possibly augment the function of, CaM I. CaBP8, contains two EF-hand domains for calcium binding and shares 70% homology with CaBP7 and 50% homology with CaM I. It negatively regulates Golgi-to-plasma membrane trafficking by interacting with PI4KB and inhibiting its activity. Both CaBP8 and CaBP7 possess a targeting mechanism that is unique amongst the CaBPs that may contribute to differential functional Ca2+-sensing by these family members.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11254 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CaBP8 antibody can be used for detection of CaBP8 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CaBP8 in 3T3 cell lysate with CaBP8 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-CaBP8 CALN1 Antibody (A11254)
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